Multilocus sequence typing scheme for the Mycobacterium abscessus complex.

Edouard Macheras; Julie Konjek; Anne-Laure Roux; Jean-Michel Thiberge; Sylvaine Bastian; Sylvia Cardoso Leão; Moises Palaci; Valérie Sivadon-Tardy; Cristina Gutierrez; Elvira Richter; Sabine Rüsch-Gerdes; Gaby E Pfyffer; Thomas Bodmer; Vincent Jarlier; Emmanuelle Cambau; Sylvain Brisse; Valérie Caro; Nalin Rastogi; Jean-Louis Gaillard; Beate Heym

doi:10.1016/j.resmic.2013.12.003

Journal articles

Multilocus sequence typing scheme for the Mycobacterium abscessus complex.

Edouard Macheras ^{1, 2} Julie Konjek ¹ Anne-Laure Roux ^{1, 3} Jean-Michel Thiberge ⁴ Sylvaine Bastian ^{1, 5} Sylvia Cardoso Leão ⁶ Moises Palaci ⁷ Valérie Sivadon-Tardy ² Cristina Gutierrez ⁸ Elvira Richter ⁹ Sabine Rüsch-Gerdes ⁹ Gaby E Pfyffer ¹⁰ Thomas Bodmer ¹¹ Vincent Jarlier ^{5, 12} Emmanuelle Cambau ¹³ Sylvain Brisse ⁴ Valérie Caro ⁴ Nalin Rastogi ¹⁴ Jean-Louis Gaillard ^{1, 2, 3} Beate Heym ^{1, 2, *}

* Corresponding author

1 2I - Infection et inflammation

2 Service de Microbiologie

3 Laboratoire de Microbiologie

4 PF8 - Génotypage des Pathogènes et Santé Publique (Plate-forme)

5 Centre National de Référence des Mycobactéries et de la Résistance aux Antituberculeux [CHU Pitié-Salpêtrière]

6 departemento de Microbiologia Immunologia e Parasitologia

7 Nucleo de Doenças Infecciosas

8 CARPHA

9 Research Center Borstel

10 Institut für Medizinische

11 Institut für Infektionskrankheiten

12 SU - Sorbonne Université

13 Laboratoire de Microbiologie

14 Institut Pasteur de la Guadeloupe

Abstract : We developed a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus sensu lato, based on the partial sequencing of seven housekeeping genes: argH, cya, glpK, gnd, murC, pta and purH. This scheme was used to characterize a collection of 227 isolates recovered between 1994 and 2010 in France, Germany, Switzerland and Brazil. We identified 100 different sequence types (STs), which were distributed into three groups on the tree obtained by concatenating the sequences of the seven housekeeping gene fragments (3576bp): the M. abscessus sensu stricto group (44 STs), the "M. massiliense" group (31 STs) and the "M. bolletii" group (25 STs). SplitTree analysis showed a degree of intergroup lateral transfers. There was also evidence of lateral transfer events involving rpoB. The most prevalent STs in our collection were ST1 (CC5; 20 isolates) and ST23 (CC3; 31 isolates). Both STs were found in Europe and Brazil, and the latter was implicated in a large post-surgical procedure outbreak in Brazil. Respiratory isolates from patients with cystic fibrosis belonged to a large variety of STs; however, ST2 was predominant in this group of patients. Our MLST scheme, publicly available at www.pasteur.fr/mlst, offers investigators a valuable typing tool for M. abscessus sensu lato in future epidemiological studies throughout the world.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Bacteriology

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https://hal-riip.archives-ouvertes.fr/pasteur-01011027
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Submitted on : Sunday, June 22, 2014 - 6:42:40 AM
Last modification on : Thursday, January 27, 2022 - 3:26:25 AM

Multilocus sequence typing scheme for the Mycobacterium abscessus complex.

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