Skip to Main content Skip to Navigation
Journal articles

Molecular epidemiology and genotyping of Mycobacterium tuberculosis isolated in Baghdad.

Abstract : Tuberculosis (TB) remains a major health problem in Iraq but the strains responsible for the epidemic have been poorly characterized. Our aim was to characterize the TB strains circulating in Bagdad (Iraq). A total of 270 Mycobacterium tuberculosis complex (MTBC) strains isolated between 2010 and 2011 from TB patients attending the Center of Chest and Respiratory diseases in Baghdad were analyzed by Spoligotyping. The analysis indicated that 94.1% of the isolates belong to known genotype clades: CAS 39.6%, ill-defined T clade 29.6%, Manu 7.4%, Haarlem 7%, Ural 4.1%, LAM 3.3%, X 0.7%, LAM7-TUR 0.7%, EAI 0.7%, S 0.7%, and unknown 5.9%. Comparison with the international multimarker database SITVIT2 showed that SIT 309 (CAS1-Delhi) and SIT1144 (T1) were the most common types. In addition, 44 strains were included in SITVIT2 database under 16 new Spoligotype International Types (SITs); of these, 6 SITs (SIT3346, SIT3497, SIT3708, SIT3790, SIT3791, and SIT3800) (n = 32 strains) were created within the present study and 10 were created after a match with an orphan in the database. By using 24-loci MIRU-VNTR-typing on a subset of 110 samples we found a high recent transmission index (RTI) of 33.6%. In conclusion, we present the first unifying framework for both epidemiology and evolutionary analysis of M. tuberculosis in Iraq.
Document type :
Journal articles
Complete list of metadatas

Cited literature [33 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01011073
Contributor : Nalin Rastogi <>
Submitted on : Sunday, June 22, 2014 - 10:27:07 PM
Last modification on : Tuesday, October 20, 2020 - 10:57:17 AM
Long-term archiving on: : Tuesday, April 11, 2017 - 8:09:17 AM

File

BMRI580981_Baghdad.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Ruqaya Mustafa Ali, Alberto Trovato, David Couvin, Amina N Al-Thwani, Emanuele Borroni, et al.. Molecular epidemiology and genotyping of Mycobacterium tuberculosis isolated in Baghdad.. BioMed Research International , Hindawi Publishing Corporation, 2014, 2014, pp.580981. ⟨10.1155/2014/580981⟩. ⟨pasteur-01011073⟩

Share

Metrics

Record views

183

Files downloads

231