Generation of eX vivo-vascularized Muscle Engineered Tissue (X-MET). - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2013

Generation of eX vivo-vascularized Muscle Engineered Tissue (X-MET).

(1) , (1) , (2) , (1) , (2, 3) , (1, 3)
1
2
3

Abstract

The object of this study was to develop an in vitro bioengineered three-dimensional vascularized skeletal muscle tissue, named eX-vivo Muscle Engineered Tissue (X-MET). This new tissue contains cells that exhibit the characteristics of differentiated myotubes, with organized contractile machinery, undifferentiated cells, and vascular cells capable of forming "vessel-like" networks. X-MET showed biomechanical properties comparable with that of adult skeletal muscles; thus it more closely mimics the cellular complexity typical of in vivo muscle tissue than myogenic cells cultured in standard monolayer conditions. Transplanted X-MET was able to mimic the activity of the excided EDL muscle, restoring the functionality of the damaged muscle. Our results suggest that X-MET is an ideal in vitro 3D muscle model that can be employed to repair muscle defects in vivo and to perform in vitro studies, limiting the use of live animals.
Fichier principal
Vignette du fichier
MusarA_-Scientific_Reports_2013.pdf (3.76 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-01025419 , version 1 (17-07-2014)

Identifiers

Cite

Silvia Carosio, Laura Barberi, Emanuele Rizzuto, Carmine Nicoletti, Zaccaria del Prete, et al.. Generation of eX vivo-vascularized Muscle Engineered Tissue (X-MET).. Scientific Reports, 2013, 3, pp.1420. ⟨10.1038/srep01420⟩. ⟨pasteur-01025419⟩

Collections

RIIP RIIP_FCB
82 View
211 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More