Skip to Main content Skip to Navigation
Journal articles

Biochemical membrane lipidomics during Drosophila development

Abstract : Lipids play critical roles in energy homeostasis, membrane structure, and signaling. Using liquid chromatography and mass spectrometry, we provide a comprehensive semiquantification of lipids during the life cycle of Drosophila melanogaster (230 glycerophospholipids, 210 sphingolipids, 6 sterols and sterol esters, and 60 glycerolipids) and obtain biological insights through this biochemical resource. First, we find a high and constant triacylglycerol-to-membrane lipid ratio during pupal stage, which is nonobvious in the absence of nutrient uptake and tissue remodeling. Second, sphingolipids undergo specific changes in headgroup (glycosylation) and tail configurations (unsaturation and hydroxylation on sphingoid base and fatty acyls, respectively), which correlate with gene expression of known (GlcT/CG6437; FA2H/ CG30502) and putative (Cyt-b5-r/CG13279) enzymes. Third, we identify a gender bias in phosphoethanolamine-ceramides as a lead for future investigation into sexual maturation. Finally, we partially characterize ghiberti, required for male meiotic cytokinesis, as a homolog of mammalian serine palmitoyltransferase.
Document type :
Journal articles
Complete list of metadatas

Cited literature [73 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01027453
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Tuesday, July 22, 2014 - 9:15:39 AM
Last modification on : Thursday, June 4, 2020 - 12:46:02 PM
Long-term archiving on: : Tuesday, April 11, 2017 - 3:57:17 PM

File

Guan_et_al.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Xue Li Guan, Gianluca Cestra, Guanghou Shui, Antje Kuhrs, Ralf B Schittenhelm, et al.. Biochemical membrane lipidomics during Drosophila development. Developmental Cell, Elsevier, 2013, 24 (1), pp.98-111. ⟨10.1016/j.devcel.2012.11.012⟩. ⟨pasteur-01027453⟩

Share

Metrics

Record views

199

Files downloads

372