The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells.

Abstract : In mammals, the biological activity of the stem/progenitor compartment sustains production of mature gametes through spermatogenesis. Spermatogonial stem cells and their progeny belong to the class of undifferentiated spermatogonia, a germ cell population found on the basal membrane of the seminiferous tubules. A large body of evidence has demonstrated that glial cell line-derived neurotrophic factor (GDNF), a Sertoli-derived factor, is essential for in vivo and in vitro stem cell self-renewal. However, the mechanisms underlying this activity are not completely understood. In this study, we show that GDNF induces dose-dependent directional migration of freshly selected undifferentiated spermatogonia, as well as germline stem cells in culture, using a Boyden chamber assay. GDNF-induced migration is dependent on the expression of the GDNF co-receptor GFRA1, as shown by migration assays performed on parental and GFRA1-transduced GC-1 spermatogonial cell lines. We found that the actin regulatory protein vasodilator-stimulated phosphoprotein (VASP) is specifically expressed in undifferentiated spermatogonia. VASP belongs to the ENA/VASP family of proteins implicated in actin-dependent processes, such as fibroblast migration, axon guidance, and cell adhesion. In intact seminiferous tubules and germline stem cell cultures, GDNF treatment up-regulates VASP in a dose-dependent fashion. These data identify a novel role for the niche-derived factor GDNF, and they suggest that GDNF may impinge on the stem/progenitor compartment, affecting the actin cytoskeleton and cell migration.
Document type :
Journal articles
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01053530
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Thursday, July 31, 2014 - 11:57:31 AM
Last modification on : Thursday, July 11, 2019 - 2:10:07 PM
Long-term archiving on: Tuesday, April 11, 2017 - 7:08:03 PM

File

Dovere_et_al.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Lisa Dovere, Stefania Fera, Margherita Grasso, Dante Lamberti, Cesare Gargioli, et al.. The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells.. PLoS ONE, Public Library of Science, 2013, 8 (4), pp.e59431. ⟨10.1371/journal.pone.0059431⟩. ⟨pasteur-01053530⟩

Share

Metrics

Record views

96

Files downloads

153