Hyaluronic acid nanohydrogels as a useful tool for BSAO immobilization in the treatment of melanoma cancer cells.

Elita Montanari (1) , Sabrina Capece (1) , Chiara Di Meo (1) , Martina Meringolo (2) , Tommasina Coviello (1) , Enzo Agostinelli (2) , Pietro Matricardi (1)

1 Department of Drug Chemistry and Technologies
2 Department of Biochemical Sciences "Rossi Fanelli"

An alternative anticancer therapy based on the use of bovine serum amine oxidase (BSAO), an enzyme that converts polyamines over-expressed in malignant cells, into hydrogen peroxide and aldehyde(s), thus inducing high cytotoxicity in cancer cells, was recently proposed. With the aim of improving the system efficacy by exploiting a nanotechnology approach, BSAO is covalently immobilized onto injectable nanohydrogels (NHs) based on cholesterol-graft-hyaluronic acid (HA-CH), a biocompatible conjugate that spontaneously leads to self-assembled structures in aqueous solutions. In this study, the physicochemical properties of the HA-CH-based NHs and the NHs cytocompatibility are reported. The properties of the NHs-BSAO system are also studied in terms of protein residual activity, both in vitro and on a model melanoma cell line.

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Cancer

Elita Montanari, Sabrina Capece, Chiara Di Meo, Martina Meringolo, Tommasina Coviello, et al.. Hyaluronic acid nanohydrogels as a useful tool for BSAO immobilization in the treatment of melanoma cancer cells.. Macromolecular Bioscience, 2013, 13 (9), pp.1185-94. ⟨10.1002/mabi.201300114⟩. ⟨pasteur-01053821⟩