M2 muscarinic receptor activation regulates Schwann cell differentiation and myelin organization. - Archive ouverte HAL Access content directly
Journal Articles Developmental Neurobiology Year : 2014

M2 muscarinic receptor activation regulates Schwann cell differentiation and myelin organization.

(1, 2) , (1, 2, 3) , (1, 2) , (1) , (1) , (4) , (5) , (6) , (1, 2)
1
2
3
4
5
6

Abstract

Glial cells express acetylcholine receptors. In particular, rat Schwann cells express different muscarinic receptor subtypes, the most abundant of which is the M2 subtype. M2 receptor activation causes a reversible arrest of the cell cycle. This negative effect on Schwann cell proliferation suggests that these cells may possibly progress into a differentiating program. In this study we analyzed the in vitro modulation, by the M2 agonist arecaidine, of transcription factors and specific signaling pathways involved in Schwann cell differentiation. The arecaidine-induced M2 receptor activation significantly upregulates transcription factors involved in the promyelinating phase (e.g., Sox10 and Krox20) and downregulates proteins involved in the maintenance of the undifferentiated state (e.g., c-jun, Notch-1, and Jagged-1). Furthermore, arecaidine stimulation significantly increases the expression of myelin proteins, which is accompanied by evident changes in cell morphology, as indicated by electron microscopy analysis, and by substantial cellular re-distribution of actin and cell adhesion molecules. Moreover, ultrastructural and morphometric analyses on sciatic nerves of M2/M4 knockout mice show numerous degenerating axons and clear alterations in myelin organization compared with wild-type mice. Therefore, our data demonstrate that acetylcholine mediates axon-glia cross talk, favoring Schwann cell progression into a differentiated myelinating phenotype and contributing to compact myelin organization.
Embargoed file
Embargoed file
Ne sera jamais visible
Loading...

Dates and versions

pasteur-01053826 , version 1 (02-08-2014)

Identifiers

Cite

Carolina Uggenti, M Egle de Stefano, Michele Costantino, Simona Loreti, Annalinda Pisano, et al.. M2 muscarinic receptor activation regulates Schwann cell differentiation and myelin organization.. Developmental Neurobiology, 2014, 74 (7), pp.676-91. ⟨10.1002/dneu.22161⟩. ⟨pasteur-01053826⟩

Collections

RIIP RIIP_FCB
68 View
3 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More