Functional dissection of the multi-domain di-heme cytochrome c(550) from Thermus thermophilus.

Sylvain Robin (1) , Marzia Arese (2) , Elena Forte (2) , Paolo Sarti (2, 3) , Olga Kolaj-Robin (1) , Alessandro Giuffrè (3) , Tewfik Soulimane (1)

1 Materials and Surface Science Institute
2 Department of Biochemical Sciences "Rossi Fanelli"
3 CNR - National Research Council of Italy | Consiglio Nazionale delle Ricerche

In bacteria, oxidation of sulfite to sulfate, the most common strategy for sulfite detoxification, is mainly accomplished by the molybdenum-containing sulfite:acceptor oxidoreductases (SORs). Bacterial SORs are very diverse proteins; they can exist as monomers or homodimers of their core subunit, as well as heterodimers with an additional cytochrome c subunit. We have previously described the homodimeric SOR from Thermus thermophilus HB8 (SOR(TTHB8)), identified its physiological electron acceptor, cytochrome c(550), and demonstrated the key role of the latter in coupling sulfite oxidation to aerobic respiration. Herein, the role of this di-heme cytochrome c was further investigated. The cytochrome was shown to be composed of two conformationally independent domains, each containing one heme moiety. Each domain was separately cloned, expressed in E. coli and purified to homogeneity. Stopped-flow experiments showed that: i) the N-terminal domain is the only one accepting electrons from SOR(TTHB8); ii) the N- and C-terminal domains are in rapid redox equilibrium and iii) both domains are able to transfer electrons further to cytochrome c(552), the physiological substrate of the ba(3) and caa(3) terminal oxidases. These findings show that cytochrome c(550) functions as a electron shuttle, without working as an electron wire with one heme acting as the electron entry and the other as the electron exit site. Although contribution of the cytochrome c(550) C-terminal domain to T. thermophilus sulfur respiration seems to be dispensable, we suggest that di-heme composition of the cytochrome physiologically enables storage of the two electrons generated from sulfite oxidation, thereof ensuring efficient contribution of sulfite detoxification to the respiratory chain-mediated energy generation.

Domaines: Biochimie, Biologie Moléculaire

Sylvain Robin, Marzia Arese, Elena Forte, Paolo Sarti, Olga Kolaj-Robin, et al.. Functional dissection of the multi-domain di-heme cytochrome c(550) from Thermus thermophilus.. PLoS ONE, 2013, 8 (1), pp.e55129. ⟨10.1371/journal.pone.0055129⟩. ⟨pasteur-01054302⟩