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The effect of ginger extract on glycoproteins of Raji cells.

Abstract : Protein glycosylation is associated with the development and progression of specific diseases, including cancers. The ginger rhizome is known to have anti-cancer and anti-fungal properties. This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells. A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C. Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software. Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out. IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance. Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans. N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit. O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein. Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins. Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
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Contributor : Zahra Zamani Connect in order to contact the contributor
Submitted on : Sunday, August 24, 2014 - 2:10:11 PM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
Long-term archiving on: : Tuesday, April 11, 2017 - 8:06:14 PM


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Zahra Zamani, Nassir Ud-Din, Haleemeh Kabini Kohan, Mehdi Kadivar, Zahra Kalyee, et al.. The effect of ginger extract on glycoproteins of Raji cells.. Pakistan Journal of Biological Sciences, 2014, 17 (2), pp.241-7. ⟨10.3923/pjbs.2014.241.247⟩. ⟨pasteur-01057590⟩



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