Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus.

Fethi Ben Abdallah (1, 2) , Rihab Lagha (2) , Ali Ellafi (2) , Abdelkader Namane (3) , Jean-Claude Rousselle (3) , Pascal Lenormand (3) , Héla Kallel (1)

1 Institut Pasteur de Tunis
2 LATVPEP - Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits
3 Protéomique (Plate-Forme)

Vibrio parahaemolyticus and V. alginolyticus, marine foodborne pathogens, were treated with UVC-radiation (240 J/m(2)) to evaluate alterations in their outer membrane protein profiles. Outer membrane protein patterns of UVC-irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Altered proteins were identified by mass spectrometry (MS and MS/MS) and analysis revealed that OmpW, OmpA, Long-chain fatty acid transport protein, Outer membrane receptor protein, Putative uncharacterized protein VP0167, Maltoporin (lamB), Polar flagellin B/D, Agglutination protein Peptidoglycan-associated lipoprotein and MltA-interacting protein MipA were appeared, thereby they can be considered as UVC-stress proteins in some vibrios. In addition, expression of OmpK decreased to non-detectable level. Furthermore, we observed a decrease or an increase in the expression level of other outer membrane proteins.

Mots clés: Vibrio; UVC; Alteration; Outer membrane proteins; Mass spectrometry

Domaines: Sciences du Vivant [q-bio]

Fethi Ben Abdallah, Rihab Lagha, Ali Ellafi, Abdelkader Namane, Jean-Claude Rousselle, et al.. Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus.. Indian J Microbiol, 2012, 52 (4), pp.660-5. ⟨10.1007/s12088-012-0299-2⟩. ⟨pasteur-01059456⟩