Whole exome sequencing identifies new causative mutations in Tunisian families with non-syndromic deafness.

Zied Riahi (1, 2) , Crystel Bonnet (3) , Rim Zainine (4) , Malek Louha (5) , Yosra Bouyacoub (1) , Nadia Laroussi (1, 2) , Mariem Chargui (1) , Rym Kefi (1) , Laurence Jonard (5) , Imen Dorboz (6) , Jean-Pierre Hardelin (3) , Sihem Belhaj Salah (4) , Jacqueline Levilliers (3) , Dominique Weil (3) , Ken Mcelreavey (7) , Odile Tanguy Boespflug (6) , Ghazi Besbes (4) , Sonia Abdelhak (1, 2) , Christine Petit (3)

1 LR11IPT05 - Laboratoire de Génomique Biomédicale et Oncogénétique - Biomedical Genomics and Oncogenetics Laboratory
2 UTM - Université de Tunis El Manar
3 Collège de France - Chaire Génétique et physiologie cellulaire
4 Hôpital La Rabta [Tunis]
5 CHU Trousseau [APHP]
6 INSERM U931, Clermont Ferrand, France
7 Génétique du Développement humain - Human developmental genetics

Identification of the causative mutations in patients affected by autosomal recessive non syndromic deafness (DFNB forms), is demanding due to genetic heterogeneity. After the exclusion of GJB2 mutations and other mutations previously reported in Tunisian deaf patients, we performed whole exome sequencing in patients affected with severe to profound deafness, from four unrelated consanguineous Tunisian families. Four biallelic non previously reported mutations were identified in three different genes: a nonsense mutation, c.208C>T (p.R70X), in LRTOMT, a missense mutation, c.5417T>C (p.L1806P), in MYO15A and two splice site mutations, c.7395+3G>A, and c.2260+2T>A, in MYO15A and TMC1 respectively. We thereby provide evidence that whole exome sequencing is a powerful, cost-effective screening tool to identify mutations causing recessive deafness in consanguineous families.

Domaines: Sciences du Vivant [q-bio]

Zied Riahi, Crystel Bonnet, Rim Zainine, Malek Louha, Yosra Bouyacoub, et al.. Whole exome sequencing identifies new causative mutations in Tunisian families with non-syndromic deafness.. PLoS ONE, 2014, 9 (6), pp.e99797. ⟨10.1371/journal.pone.0099797⟩. ⟨pasteur-01060192⟩