Skip to Main content Skip to Navigation
Journal articles

Glycoprotein 60 diversity in Cryptosporidium parvum causing human and cattle cryptosporidiosis in the rural region of Northern Tunisia.

Abstract : The zoonotic potential of Cryptosporidium parvum was studied in an extensive cattle farming region of northern Tunisia. Seventy fecal samples from pre-weaning calves and 403 fecal samples from children were examined by microscopy after modified Ziehl-Neelsen (MZN) staining. Positive Cryptosporidium specimens were identified at a species level using an 18S rRNA nested polymerase chain reaction (PCR) followed by an Restriction Fragment Length Polymorphism (RFLP) analysis. C. parvum isolates were subgenotyped by sequence analysis of the glycoprotein 60 (gp60) gene. Among calf samples, 14 samples were positive by MZN method. C. parvum was identified in all cases. Twelve parvum isolates (85.7%) belonged to family subtype IIa. Subtype IIaA15G2R1 was more prevalent (50%). Two C. parvum isolates corresponded to the IIdA16G1 subtype. Seven human samples were positive by MZN method. C. parvum and C. meleagridis were identified in four and three cases, respectively. Intraspecific characterization of C. parvum identified two subtypes, the IIaA15G2R1 and the IIdA16G1, also found in calves.
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-01061049
Contributor : Institut Pasteur Tunis <>
Submitted on : Friday, September 5, 2014 - 9:40:20 AM
Last modification on : Monday, October 8, 2018 - 5:44:08 PM

Links full text

Identifiers

Collections

Citation

Ikram Rahmouni, Rym Essid, Karim Aoun, Aïda Bouratbine. Glycoprotein 60 diversity in Cryptosporidium parvum causing human and cattle cryptosporidiosis in the rural region of Northern Tunisia.. American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2014, 90 (2), pp.346-50. ⟨10.4269/ajtmh.13-0522⟩. ⟨pasteur-01061049⟩

Share

Metrics

Record views

158