C. Github and . Repository,

T. Smith, H. P. Beck, A. Kitua, S. Mwankusye, I. Felger et al., Age dependence of the multiplicity of Plasmodium falciparum infections and of other malariological indices in an area of high endemicity, Trans R Soc Trop Med Hyg, vol.93, pp.15-20, 1999.

I. Felger, T. Smith, D. Edoh, A. Kitua, P. Alonso et al., Multiple Plasmodium falciparum infections in Tanzanian infants, Trans R Soc Trop Med Hyg, vol.93, issue.1, pp.29-34, 1999.

A. Guerra-neira, J. M. Rubio, J. R. Royo, J. C. Ortega, A. S. Auñón et al., Plasmodium diversity in non-malaria individuals from the Bioko Island in Equatorial Guinea (West Central-Africa), Int J Health Geogr, vol.5, p.27, 2006.

F. Ntoumi, H. Contamin, C. Rogier, S. Bonnefoy, J. F. Trape et al., Age-dependent carriage of multiple Plasmodium falciparum merozoite surface antigen-2 alleles in asymptomatic malaria infections, Am J Trop Med Hyg, vol.52, pp.81-89, 1995.

H. P. Beck, I. Felger, W. Huber, S. Steiger, T. Smith et al., Analysis of multiple Plasmodium falciparum infections in Tanzanian children during the phase III trial of the malaria vaccine SPf66, J Infect Dis, vol.175, pp.921-927, 1997.

S. Enosse, C. Dobaño, D. Quelhas, J. J. Aponte, M. Lievens et al., S/AS02A malaria vaccine does not induce parasite CSP T cell epitope selection and reduces multiplicity of infection, PLoS Clin Trials, vol.1, p.5, 2006.

O. H. Branch, S. Takala, S. Kariuki, B. L. Nahlen, M. Kolczak et al., Plasmodium falciparum genotypes, low complexity of infection, and resistance to subsequent malaria in participants in the Asembo Bay Cohort Project, Infect Immun, vol.69, pp.7783-92, 2001.

A. Ofosu-okyere, M. J. Mackinnon, M. P. Sowa, K. A. Koram, F. Nkrumah et al., Novel Plasmodium falciparum clones and rising clone multiplicities are associated with the increase in malaria morbidity in Ghanaian children during the transition into the high transmission season, Parasitology, vol.123, issue.2, pp.113-136, 2001.

F. Al-yaman, B. Genton, J. C. Reeder, R. F. Anders, T. Smith et al., Reduced risk of clinical malaria in children infected with multiple clones of Plasmodium falciparum in a highly endemic area: a prospective community study, Trans R Soc Trop Med Hyg, vol.91, pp.602-607, 1997.

A. Färnert, I. Rooth, . Svensson, G. Snounou, and A. Björkman, Complexity of Plasmodium falciparum infections is consistent over time and protects against clinical disease in Tanzanian children, J Infect Dis, vol.179, pp.989-95, 1999.

D. A. Müller, J. D. Charlwood, I. Felger, C. Ferreira, D. Rosario et al., Prospective risk of morbidity in relation to multiplicity of infection with Plasmodium falciparum in São Tomé, Acta Trop, vol.78, pp.155-62, 2001.

M. S. Kiwuwa, U. Ribacke, K. Moll, J. Byarugaba, K. Lundblom et al., Genetic diversity of Plasmodium falciparum infections in mild and severe malaria of children from Kampala, Uganda. Parasitol Res, vol.112, pp.1691-700, 2013.

M. Vafa, M. Troye-blomberg, J. Anchang, and A. Garcia, Migot-Nabias F. Multiplicity of Plasmodium falciparum infection in asymptomatic children in Senegal: relation to transmission, age and erythrocyte variants, Malar J, vol.7, p.17, 2008.

D. Arnot, Unstable malaria in Sudan: the influence of the dry season. Clone multiplicity of Plasmodium falciparum infections in individuals exposed to variable levels of disease transmission, Trans R Soc Trop Med Hyg, vol.92, pp.580-585, 1998.

H. A. Babiker, Unstable malaria in Sudan: the influence of the dry season. Plasmodium falciparum population in the unstable malaria area of eastern Sudan is stable and genetically complex, Trans R Soc Trop Med Hyg, vol.92, pp.585-594, 1998.

S. C. Nkhoma, S. Nair, S. Al-saai, A. E. Mcgready, R. Phyo et al., Population genetic correlates of declining transmission in a human pathogen, Mol Ecol, vol.22, pp.273-85, 2013.

K. Tanabe, N. Sakihama, O. Kaneko, A. Saito-ito, and M. Kimura, A PCR method for molecular epidemiology of Plasmodium falciparum Msp-1, Tokai J Exp Clin Med, vol.23, pp.375-81, 1998.

H. Contamin, T. Fandeur, S. Bonnefoy, F. Skouri, F. Ntoumi et al., PCR typing of field isolates of Plasmodium falciparum, J Clin Microbiol, vol.33, pp.944-51, 1995.
URL : https://hal.archives-ouvertes.fr/pasteur-01472764

W. G. Hill and H. A. Babiker, Estimation of numbers of malaria clones in blood samples, Proc Biol Sci, vol.262, pp.249-57, 1995.

S. Auburn, S. Campino, O. Miotto, A. A. Djimde, I. Zongo et al., Characterization of within-host Plasmodium falciparum diversity using next-generation sequence data, PLoS One, vol.7, p.32891, 2012.

S. A. Assefa, M. D. Preston, S. Campino, H. Ocholla, C. J. Sutherland et al., estMOI: estimating multiplicity of infection using parasite deep sequencing data, Bioinformatics, vol.30, pp.1292-1296, 2014.

R. Daniels, S. K. Volkman, D. A. Milner, M. N. Neafsey, D. E. Park et al., A general SNP-based molecular barcode for Plasmodium falciparum identification and tracking, Malar J, vol.7, p.223, 2008.

D. F. Echeverry, S. Nair, L. Osorio, S. Menon, C. Murillo et al., Long term persistence of clonal malaria parasite Plasmodium falciparum lineages in the Colombian Pacific region, BMC Genet, vol.14, issue.2, 2013.

R. Daniels, H. Chang, P. D. Séne, D. C. Park, D. E. Neafsey et al., Genetic surveillance detects both clonal and epidemic transmission of malaria following enhanced intervention in Senegal, PLoS One, vol.8, p.60780, 2013.

S. Campino, S. Auburn, K. Kivinen, I. Zongo, J. Ouedraogo et al., Population genetic analysis of Plasmodium falciparum parasites using a customized Illumina GoldenGate genotyping assay, PLoS One, vol.6, p.20251, 2011.

M. D. Preston, S. Campino, S. A. Assefa, D. F. Echeverry, H. Ocholla et al., A barcode of organellar genome polymorphisms identifies the geographic origin of Plasmodium falciparum strains, Nat Commun, vol.5, p.4052, 2014.

S. K. Volkman, P. C. Sabeti, D. Decaprio, D. E. Neafsey, S. F. Schaffner et al., A genome-wide map of diversity in Plasmodium falciparum, Nat Genet, vol.39, pp.113-122, 2007.

P. Orjuela-sánchez, N. D. Karunaweera, M. Da-silva-nunes, N. S. Da-silva, K. Scopel et al., Single-nucleotide polymorphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax: prospects for genome-wide association studies, BMC Genet, vol.11, p.65, 2010.

P. Druilhe, P. Daubersies, J. Patarapotikul, C. Gentil, L. Chene et al., A primary malarial infection is composed of a very wide range of genetically diverse but related parasites, J Clin Invest, vol.101, pp.2008-2024, 1998.

S. C. Nkhoma, S. Nair, I. H. Cheeseman, C. Rohr-allegrini, S. Singlam et al., Close kinship within multiple-genotype malaria parasite infections, Proc Biol Sci, 2012.

S. Nair, S. C. Nkhoma, D. Serre, P. A. Zimmerman, K. Gorena et al., Single-cell genomics for dissection of complex malaria infections, Genome Res, vol.24, pp.1028-1066, 2014.

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