Protective effects of caffeic acid and caffeic acid phenethyl ester against acrolein-induced neurotoxicity in HT22 mouse hippocampal cells

Ying Huang 1 Minghua Jin 1 Rongbiao Pi 1 Jun Zhang 1 Meihui Chen 1 Ying Ouyang 1 Anmin Liu 1 Xiaojuan Chao 1 Peiqing Liu 1 Jun Liu 1 Charles Ramassamy 2 Jian Qin 1
1 SYSU - Sun Yat-Sen University
2 INRS-IAF - Institut Armand Frappier
Abstract : Acrolein-induced oxidative stress is hypothesized to involve in the etiology of Alzheimer's disease (AD). Caffeic acid (CA) and caffeic acid phenethyl ester (CAPE) have antioxidative and neuroprotective properties. The present study investigated the protective effects of CA/CAPE on acrolein-induced oxidative neuronal toxicity. HT22 mouse hippocampal cells were pretreated with CA/CAPE and then exposed to acrolein. Cell viability, intracellular reactive oxygen species (ROS), and glutathione (GSH) level were measured. MAPKs and Akt/GSK3β signaling proteins as well as α/β-secretase of amyloid protein precursor were assayed by Western blotting. Pretreatment with CA/CAPE significantly attenuated acrolein-induced neurotoxicity, ROS accumulation, and GSH depletion. Further study suggested that CA/CAPE showed protective effects against acrolein by modulating MAPKs and Akt/GSK3β signaling pathways. Moreover, CA/CAPE restored the changes of β-secretase (BACE-1) and/or activation of α-secretase (ADAM-10) induced by acrolein. These findings suggest that CA/CAPE may provide a promising approach for the treatment of acrolein-related neurodegenerative diseases, such as AD.
Keywords : Alzheimer's disease Caffeic acid Acrolein Oxidative stress
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Journal articles
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https://hal-riip.archives-ouvertes.fr/pasteur-01131056
Contributor : Michel Courcelles <>
Submitted on : Thursday, March 12, 2015 - 7:34:29 PM
Last modification on : Friday, July 12, 2019 - 8:10:02 PM

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Ying Huang, Minghua Jin, Rongbiao Pi, Jun Zhang, Meihui Chen, et al.. Protective effects of caffeic acid and caffeic acid phenethyl ester against acrolein-induced neurotoxicity in HT22 mouse hippocampal cells. Neuroscience Letters, Elsevier, 2013, 535, pp.146 - 151. ⟨10.1016/j.neulet.2012.12.051⟩. ⟨pasteur-01131056⟩

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