 |
Journal articles
Melatonin: the watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis.
Abstract : Human placenta produces melatonin and expresses its receptors. We propose that melatonin, an antioxidant, protects the human placenta against hypoxia/reoxygenation (H/R)-induced damage. Primary term villous cytotrophoblasts were cultured under normoxia (8% O2) with or without 1mM melatonin for 72h to induce differentiation into the syncytiotrophoblast. The cells were then cultured for an additional 22h under normoxia or subjected to hypoxia (0.5% O2) for 4h followed by 18h reoxygenation (8% O2) with or without melatonin. H/R induced oxidative stress, which activated the Bax/Bcl-2 mitochondrial apoptosis pathway and the downstream fragmentation of DNA. Villous trophoblast treatment with melatonin reversed all the negative effects induced by H/R to normoxic levels. This study shows that melatonin protects the villous trophoblast against H/R-induced oxidative stress and apoptosis and suggests a potential preventive and therapeutic use of this indolamine in pregnancy complications characterized by syncytiotrophoblast survival alteration.
https://hal-riip.archives-ouvertes.fr/pasteur-01131943
Contributor : Michel Courcelles Connect in order to contact the contributor
Submitted on : Monday, March 16, 2015 - 2:14:58 PM
Last modification on : Wednesday, December 8, 2021 - 2:48:07 PM
Contributor : Michel Courcelles Connect in order to contact the contributor
Submitted on : Monday, March 16, 2015 - 2:14:58 PM
Last modification on : Wednesday, December 8, 2021 - 2:48:07 PM