Expression strategy of Aedes albopictus densovirus.

Hanh T Pham (1) , Françoise-Xavière Jousset (2) , Jonathan Perreault (1) , Hiroko Shike (3) , Jozsef Szelei (1) , Max Bergoin (1) , Peter Tijssen (1)

1 INRS-IAF - Institut Armand Frappier
2 LPC - Laboratoire de Pathologie Comparée
3 Division of Clinical Pathology, Milton S. Hershey Medical Center

The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.

Domaines: Sciences du Vivant [q-bio]; Virologie; Parasitologie

Hanh T Pham, Françoise-Xavière Jousset, Jonathan Perreault, Hiroko Shike, Jozsef Szelei, et al.. Expression strategy of Aedes albopictus densovirus.. Journal of Virology, 2013, 87 (17), pp.9928-32. ⟨10.1128/JVI.01259-13⟩. ⟨pasteur-01132101⟩