Convergent evolution of hyperswarming leads to impaired biofilm formation in pathogenic bacteria.

Abstract : Most bacteria in nature live in surface-associated communities rather than planktonic populations. Nonetheless, how surface-associated environments shape bacterial evolutionary adaptation remains poorly understood. Here, we show that subjecting Pseudomonas aeruginosa to repeated rounds of swarming, a collective form of surface migration, drives remarkable parallel evolution toward a hyperswarmer phenotype. In all independently evolved hyperswarmers, the reproducible hyperswarming phenotype is caused by parallel point mutations in a flagellar synthesis regulator, FleN, which locks the naturally monoflagellated bacteria in a multiflagellated state and confers a growth rate-independent advantage in swarming. Although hyperswarmers outcompete the ancestral strain in swarming competitions, they are strongly outcompeted in biofilm formation, which is an essential trait for P. aeruginosa in environmental and clinical settings. The finding that evolution in swarming colonies reliably produces evolution of poor biofilm formers supports the existence of an evolutionary trade-off between motility and biofilm formation.
Document type :
Journal articles
Complete list of metadatas

Cited literature [80 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01133101
Contributor : Michel Courcelles <>
Submitted on : Wednesday, March 18, 2015 - 3:10:28 PM
Last modification on : Tuesday, August 13, 2019 - 3:02:16 PM
Long-term archiving on : Monday, April 17, 2017 - 6:40:22 PM

File

1-s2.0-S2211124713003884-main....
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Dave van Ditmarsch, Kerry E Boyle, Hassan Sakhtah, Jennifer E Oyler, Carey D Nadell, et al.. Convergent evolution of hyperswarming leads to impaired biofilm formation in pathogenic bacteria.. Plant Cell Reports, Springer Verlag, 2013, 4 (4), pp.697-708. ⟨10.1016/j.celrep.2013.07.026⟩. ⟨pasteur-01133101⟩

Share

Metrics

Record views

66

Files downloads

143