Genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent.

Avik Khan 1 Stéphane Salmieri 1 Carole Fraschini 2 Jean Bouchard 2 Bernard Riedl 3 Monique Lacroix 1, *
* Corresponding author
1 INRS-IAF - Institut Armand Frappier
2 Département des Sciences du Bois et de la Forêt, Faculté de Foresterie, Géographie et Géomatique
3 FPInnovations
Abstract : Cellulose nanocrystal (CNC) reinforced chitosan based antimicrobial films were prepared by immobilizing nisin on the surface of the films. Nanocomposite films containing 18.65 μg/cm(2) of nisin reduced the count of L. monocytogenes by 6.73 log CFU/g, compared to the control meat samples (8.54 log CFU/g) during storage at 4 °C in a Ready-To-Eat (RTE) meat system. Film formulations containing 9.33 μg/cm(2) of nisin increased the lag phase of L. monocytogenes on meat by more than 21 days, whereas formulations with 18.65 μg/cm(2) completely inhibited the growth of L. monocytogenes during storage. Genipin was used to cross-link and protect the activity of nisin during storage. Nanocomposite films cross-linked with 0.05% w/v genipin exhibited the highest bioactivity (10.89 μg/cm(2)) during the storage experiment, as compared to that of the un-cross-linked films (7.23 μg/cm(2)). Genipin cross-linked films were able to reduce the growth rate of L. monocytogenes on ham samples by 21% as compared to the un-cross-linked films. Spectroscopic analysis confirmed the formation of genipin-nisin-chitosan heterocyclic cross-linked network. Genipin cross-linked films also improved the swelling, water solubility, and mechanical properties of the nanocomposite films.
Keywords : surface modification nisin genipin chitosan antimicrobial films Listeria monocytogenes
Document type :
Journal articles
Complete list of metadatas
https://hal-riip.archives-ouvertes.fr/pasteur-01135762
Contributor : Michel Courcelles <>
Submitted on : Wednesday, March 25, 2015 - 7:58:06 PM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM

Links full text

openaccess

Identifiers

Collections

RIIP | INRS-IAF

Citation

Avik Khan, Stéphane Salmieri, Carole Fraschini, Jean Bouchard, Bernard Riedl, et al.. Genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent.. ACS Appl Mater Interfaces, 2014, 6 (17), pp.15232-42. ⟨10.1021/am503564m⟩. ⟨pasteur-01135762⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

120

Contact

Informations

CCSD