Genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent.

Abstract : Cellulose nanocrystal (CNC) reinforced chitosan based antimicrobial films were prepared by immobilizing nisin on the surface of the films. Nanocomposite films containing 18.65 μg/cm(2) of nisin reduced the count of L. monocytogenes by 6.73 log CFU/g, compared to the control meat samples (8.54 log CFU/g) during storage at 4 °C in a Ready-To-Eat (RTE) meat system. Film formulations containing 9.33 μg/cm(2) of nisin increased the lag phase of L. monocytogenes on meat by more than 21 days, whereas formulations with 18.65 μg/cm(2) completely inhibited the growth of L. monocytogenes during storage. Genipin was used to cross-link and protect the activity of nisin during storage. Nanocomposite films cross-linked with 0.05% w/v genipin exhibited the highest bioactivity (10.89 μg/cm(2)) during the storage experiment, as compared to that of the un-cross-linked films (7.23 μg/cm(2)). Genipin cross-linked films were able to reduce the growth rate of L. monocytogenes on ham samples by 21% as compared to the un-cross-linked films. Spectroscopic analysis confirmed the formation of genipin-nisin-chitosan heterocyclic cross-linked network. Genipin cross-linked films also improved the swelling, water solubility, and mechanical properties of the nanocomposite films.
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Submitted on : Wednesday, March 25, 2015 - 7:58:06 PM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM

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Avik Khan, Stéphane Salmieri, Carole Fraschini, Jean Bouchard, Bernard Riedl, et al.. Genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent.. ACS Appl Mater Interfaces, 2014, 6 (17), pp.15232-42. ⟨10.1021/am503564m⟩. ⟨pasteur-01135762⟩

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