Interaction between silver nanoparticles of 20 nm (AgNP 20 ) and human neutrophils: induction of apoptosis and inhibition of de novo protein synthesis by AgNP 20 aggregates - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Journal of Applied Toxicology Année : 2014

Interaction between silver nanoparticles of 20 nm (AgNP 20 ) and human neutrophils: induction of apoptosis and inhibition of de novo protein synthesis by AgNP 20 aggregates

Résumé

Cytotoxic and proinflammatory properties of silver nanoparticles (AgNPs) have been reported in few studies but the direct interaction between AgNPs and neutrophils, which play a key role in inflammation, has never been documented. Here, we examined the role of AgNPs with a starting size of 20 nm (AgNP 20) in human neutrophils. Using dynamic light scattering for the characterization of NPs suspended under identical conditions to those used for in vitro experiments, we found that, at 10 μg ml –1 , 92% of AgNP 20 possess a diameter of 17.1 nm but, at 100 μg ml –1 , a tri-modal size distribution with large aggregates was observed (> 500 nm). Neutrophil cell size increased when treated with AgNP 20 and transmission electronic microscopy experiments revealed that AgNP 20 can rapidly interact with the cell membrane, penetrate neutrophils, localize in vacuole-like structures, and be randomly distributed in the cytosol after 24 h. Treatment with 100 μg ml –1 AgNP 20 for 24 h (but not 10 μg ml –1) increased the neutrophil apoptotic rate and inhibited de novo protein synthesis. We conclude that AgNP 20 induced apoptosis and can act as potent inhibitors of de novo protein synthesis at 100, but not 10 μg ml –1 in human neutrophils.
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hal-01197016 , version 1 (14-09-2015)

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Michelle Poirier, Jean-Christophe Simard, Francis Antoine, Denis Girard. Interaction between silver nanoparticles of 20 nm (AgNP 20 ) and human neutrophils: induction of apoptosis and inhibition of de novo protein synthesis by AgNP 20 aggregates. Journal of Applied Toxicology, 2014, 34 (4), pp.404-412. ⟨10.1002/jat.2956⟩. ⟨hal-01197016⟩

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