Skip to Main content Skip to Navigation
Journal articles

Visualization of mouse neuronal ganglia infected by Herpes Simplex Virus 1 (HSV-1) using multimodal non-linear optical microscopy.

Abstract : Herpes simplex virus 1 (HSV-1) is a neurotropic virus that causes skin lesions and goes on to enter a latent state in neurons of the trigeminal ganglia. Following stress, the virus may reactivate from latency leading to recurrent lesions. The in situ study of neuronal infections by HSV-1 is critical to understanding the mechanisms involved in the biology of this virus and how it causes disease; however, this normally requires fixation and sectioning of the target tissues followed by treatment with contrast agents to visualize key structures, which can lead to artifacts. To further our ability to study HSV-1 neuropathogenesis, we have generated a recombinant virus expressing a second generation red fluorescent protein (mCherry), which behaves like the parental virus in vivo. By optimizing the application of a multimodal non-linear optical microscopy platform, we have successfully visualized in unsectioned trigeminal ganglia of mice both infected cells by two-photon fluorescence microscopy, and myelinated axons of uninfected surrounding cells by coherent anti-Stokes Raman scattering (CARS) microscopy. These results represent the first report of CARS microscopy being combined with 2-photon fluorescence microscopy to visualize virus-infected cells deep within unsectioned explanted tissue, and demonstrate the application of multimodal non-linear optical microscopy for high spatial resolution biological imaging of tissues without the use of stains or fixatives.
Complete list of metadatas

Cited literature [51 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01136190
Contributor : Michel Courcelles <>
Submitted on : Thursday, March 26, 2015 - 4:43:20 PM
Last modification on : Friday, March 27, 2020 - 3:18:03 PM
Long-term archiving on: : Thursday, July 2, 2015 - 8:07:24 AM

File

journal.pone.0105103.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Pierre-Alexandre Rochette, Mathieu Laliberté, Antony Bertrand-Grenier, Marie-Andrée Houle, Marie-Claire Blache, et al.. Visualization of mouse neuronal ganglia infected by Herpes Simplex Virus 1 (HSV-1) using multimodal non-linear optical microscopy.. PLoS ONE, Public Library of Science, 2014, 9 (8), pp.e105103. ⟨10.1371/journal.pone.0105103⟩. ⟨pasteur-01136190⟩

Share

Metrics

Record views

423

Files downloads

621