Visualization of mouse neuronal ganglia infected by Herpes Simplex Virus 1 (HSV-1) using multimodal non-linear optical microscopy.

Pierre-Alexandre Rochette 1 Mathieu Laliberté 2 Antony Bertrand-Grenier 2 Marie-Andrée Houle 2 Marie-Claire Blache 2 François Légaré 2 Angela Pearson 1, *
* Auteur correspondant
1 INRS-IAF - Institut Armand Frappier
2 EMT-INRS - Énergie Matériaux Télécommunications - INRS
Abstract : Herpes simplex virus 1 (HSV-1) is a neurotropic virus that causes skin lesions and goes on to enter a latent state in neurons of the trigeminal ganglia. Following stress, the virus may reactivate from latency leading to recurrent lesions. The in situ study of neuronal infections by HSV-1 is critical to understanding the mechanisms involved in the biology of this virus and how it causes disease; however, this normally requires fixation and sectioning of the target tissues followed by treatment with contrast agents to visualize key structures, which can lead to artifacts. To further our ability to study HSV-1 neuropathogenesis, we have generated a recombinant virus expressing a second generation red fluorescent protein (mCherry), which behaves like the parental virus in vivo. By optimizing the application of a multimodal non-linear optical microscopy platform, we have successfully visualized in unsectioned trigeminal ganglia of mice both infected cells by two-photon fluorescence microscopy, and myelinated axons of uninfected surrounding cells by coherent anti-Stokes Raman scattering (CARS) microscopy. These results represent the first report of CARS microscopy being combined with 2-photon fluorescence microscopy to visualize virus-infected cells deep within unsectioned explanted tissue, and demonstrate the application of multimodal non-linear optical microscopy for high spatial resolution biological imaging of tissues without the use of stains or fixatives.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2014, 9 (8), pp.e105103. 〈10.1371/journal.pone.0105103〉
Liste complète des métadonnées

Littérature citée [51 références]  Voir  Masquer  Télécharger
https://hal-riip.archives-ouvertes.fr/pasteur-01136190
Contributeur : Michel Courcelles <>
Soumis le : jeudi 26 mars 2015 - 16:43:20
Dernière modification le : lundi 8 octobre 2018 - 17:44:05
Document(s) archivé(s) le : jeudi 2 juillet 2015 - 08:07:24

Fichier

journal.pone.0105103.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

RIIP | INRS-IAF

Citation

Pierre-Alexandre Rochette, Mathieu Laliberté, Antony Bertrand-Grenier, Marie-Andrée Houle, Marie-Claire Blache, et al.. Visualization of mouse neuronal ganglia infected by Herpes Simplex Virus 1 (HSV-1) using multimodal non-linear optical microscopy.. PLoS ONE, Public Library of Science, 2014, 9 (8), pp.e105103. 〈10.1371/journal.pone.0105103〉. 〈pasteur-01136190〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

196

Téléchargements de fichiers

91

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD