Effect of γ-irradiation on gene expression of heat shock proteins in the foodborne pathogen Escherichia coli O157:H7.

Abstract : The expression levels of seven genes (clpB, dnaK, groES, grpE, htpG, htpX and ibpB) encoding heat shock proteins (HSP) in Escherichia coli O157:H7 (E. coli) gamma irradiated was investigated. Timing impact of post-irradiated RNA extraction on the expression levels of these seven genes was also studied at a dose damaging the bacterial cells (0.4 kGy). Bacterial samples were γ-irradiated at 0.4 kGy and at a lethal dose of 1.3 kGy. RNA was extracted at 0 min post irradiation for both irradiation doses and at 15, 30, 60, 90 or 120 min post-irradiation at the dose damaging the cells. Quantification of the gene expression was performed using quantitative real-time polymerase chain reaction (q-RT-PCR). The expression of genes encoding HSP was a very dynamic process evolving rapidly when E. coli cells were irradiated at 0.4 kGy. Notably, groES, grpE and ibpB were more up- regulated at 1.3 kGy than those at 0.4 kGy. For the seven genes studied there were more damaged proteins during irradiation at the lethal dose and this dose causes increased expression in HSP which contributes to damage reparation. Expression patterns of genes encoding HSP in E. coli treated by γ-irradiation are different from those treated by heat shock.
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International Journal of Radiation Biology, Informa Healthcare, 2014, 90 (3), pp.268-73. 〈10.3109/09553002.2014.859766〉
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Soumis le : jeudi 26 mars 2015 - 20:42:48
Dernière modification le : lundi 5 février 2018 - 15:22:10

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Karine Trudeau, Khanh Dang Vu, Éric Déziel, François Shareck, Monique Lacroix. Effect of γ-irradiation on gene expression of heat shock proteins in the foodborne pathogen Escherichia coli O157:H7.. International Journal of Radiation Biology, Informa Healthcare, 2014, 90 (3), pp.268-73. 〈10.3109/09553002.2014.859766〉. 〈pasteur-01136304〉

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