Prevalence of Latin-American-Mediterranean genetic family in population structure of Mycobacterium tuberculosis in Bulgaria

Abstract : Tuberculosis (TB) control remains an important public health priority for Bulgaria. The population structure of Mycobacterium tuberculosis is clonal and certain genetic families of this species (e.g., Latin-American-Mediterranean [LAM]) have attracted more attention due to their global dissemination and/or particular pathogenic properties, e.g., association with multidrug resistance (MDR). The aim of this study was to evaluate the prevalence of the M. tuberculosis LAM family in Bulgaria based on the use of different molecular markers. A total of 101 previously spoligotyped M. tuberculosis strains were studied by LAM-specific PCR assay to detect an insertion of IS6110 in the specific genome region. On the whole, clear-cut results were obtained for most strains; spoligotype-based family was reassigned in some of them. At the same time, double bands were amplified in some cases and warrant further validation studies of this method. The higher MDR rate among LAM versus other genotype isolates was observed (P = 0.04). In conclusion, these results suggest a low (<4%) prevalence rate of LAM in Bulgaria (that is similar to its Balkan neighbors) and highlight the importance of using robust markers for correct detection of the LAM family.
Type de document :
Article dans une revue
International Journal of Mycobacteriology, 2015, 4, [Epub ahead of print]. 〈10.1016/j.ijmyco.2015.04.003〉
Liste complète des métadonnées

https://hal-riip.archives-ouvertes.fr/pasteur-01154025
Contributeur : Nalin Rastogi <>
Soumis le : mercredi 20 mai 2015 - 23:40:10
Dernière modification le : lundi 5 février 2018 - 15:22:11
Document(s) archivé(s) le : mardi 15 septembre 2015 - 06:35:30

Fichier

IJMYCO2015_Bulgaria_with_Suppl...
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale - Pas de modification 4.0 International License

Identifiants

Citation

Violeta Valcheva, Nalin Rastogi, Igor Mokrousov. Prevalence of Latin-American-Mediterranean genetic family in population structure of Mycobacterium tuberculosis in Bulgaria. International Journal of Mycobacteriology, 2015, 4, [Epub ahead of print]. 〈10.1016/j.ijmyco.2015.04.003〉. 〈pasteur-01154025〉

Partager

Métriques

Consultations de la notice

150

Téléchargements de fichiers

42