Skip to Main content Skip to Navigation
Journal articles

Poly(ADP-ribosyl)ation is involved in the epigenetic control of TET1 gene transcription.

Abstract : TET enzymes are the epigenetic factors involved in the formation of the sixth DNA base 5-hydroxymethylcytosine, whose deregulation has been associated with tumorigenesis. In particular, TET1 acts as tumor suppressor preventing cell proliferation and tumor metastasis and it has frequently been found down-regulated in cancer. Thus, considering the importance of a tight control of TET1 expression, the epigenetic mechanisms involved in the transcriptional regulation of TET1 gene are here investigated. The involvement of poly(ADP-ribosyl)ation in the control of DNA and histone methylation on TET1 gene was examined. PARP activity is able to positively regulate TET1 expression maintaining a permissive chromatin state characterized by DNA hypomethylation of TET1 CpG island as well as high levels of H3K4 trimethylation. These epigenetic modifications were affected by PAR depletion causing TET1 down-regulation and in turn reduced recruitment of TET1 protein on HOXA9 target gene. In conclusion, this work shows that PARP activity is a transcriptional regulator of TET1 gene through the control of epigenetic events and it suggests that deregulation of these mechanisms could account for TET1 repression in cancer.
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-01163733
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Monday, June 15, 2015 - 2:15:29 PM
Last modification on : Wednesday, September 25, 2019 - 11:54:55 AM
Document(s) archivé(s) le : Tuesday, September 15, 2015 - 2:31:31 PM

File

oncotarget-05-10356.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Fabio Ciccarone, Elisabetta Valentini, Maria Giulia Bacalini, Michele Zampieri, Roberta Calabrese, et al.. Poly(ADP-ribosyl)ation is involved in the epigenetic control of TET1 gene transcription.. Oncotarget, Impact journals, 2014, 5 (21), pp.10356-67. ⟨10.18632/oncotarget.1905⟩. ⟨pasteur-01163733⟩

Share

Metrics

Record views

257

Files downloads

244