The mechanism of binding of the second PDZ domain from the Protein Tyrosine Phosphatase-BL to the Adenomatous Polyposis Coli tumor suppressor.

Eva Di Silvio 1 Daniela Bonetti 1 Angelo Toto 1 Angela Morrone 1 Stefano Gianni 1, 2, *
* Auteur correspondant
1 Department of Biochemical Sciences
2 Department of Chemistry [Cambridge, UK]
Abstract : Many biological processes are regulated by the interaction between protein domains and their corresponding binding partners. The PDZ domain is one of the most common protein-protein interaction modules in mammalian cells, whose role is to bind C-terminal sequences of specific targets. The second PDZ domain from the Protein Tyrosine Phosphatase-BL (PDZ2) binds to the C-terminal of Adenomatous Polyposis Coli protein (APC), one of the major tumor suppressor whose task is to regulate cell adhesion and proliferation. Here, we present a detailed kinetics analysis of the interaction between PDZ2 domain and a peptide mimicking the PDZ binding motif of APC. By analyzing data obtained at different experimental conditions, we propose a plausible mechanism for binding. Furthermore, a comparison between the dissociation rate constant measured by different methodologies allow us to identify an additional kinetic step, which is likely to arise from a conformational change of PDZ2 occurring after binding. The data are discussed on the light of previous work on PDZ domains.
Type de document :
Article dans une revue
Protein Engineering, Design and Selection, Oxford University Press (OUP), 2014, 27 (8), pp.249-53. 〈10.1093/protein/gzu022〉
Liste complète des métadonnées

Littérature citée [28 références]  Voir  Masquer  Télécharger
https://hal-riip.archives-ouvertes.fr/pasteur-01181359
Contributeur : Istituto Pasteur Fondazione Cenci Bolognetti <>
Soumis le : jeudi 30 juillet 2015 - 09:28:20
Dernière modification le : lundi 5 février 2018 - 15:22:13
Document(s) archivé(s) le : samedi 31 octobre 2015 - 10:21:59

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

RIIP | RIIP_FCB

Citation

Eva Di Silvio, Daniela Bonetti, Angelo Toto, Angela Morrone, Stefano Gianni. The mechanism of binding of the second PDZ domain from the Protein Tyrosine Phosphatase-BL to the Adenomatous Polyposis Coli tumor suppressor.. Protein Engineering, Design and Selection, Oxford University Press (OUP), 2014, 27 (8), pp.249-53. 〈10.1093/protein/gzu022〉. 〈pasteur-01181359〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

91

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD