Conserved nucleation sites reinforce the significance of Phi value analysis in protein-folding studies.

Abstract : The only experimental strategy to address the structure of folding transition states, the so-called Φ value analysis, relies on the synergy between site directed mutagenesis and the measurement of reaction kinetics. Despite its importance, the Φ value analysis has been often criticized and its power to pinpoint structural information has been questioned. In this hypothesis, we demonstrate that comparing the Φ values between proteins not only allows highlighting the robustness of folding pathways but also provides per se a strong validation of the method.
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Stefano Gianni, Per Jemth. Conserved nucleation sites reinforce the significance of Phi value analysis in protein-folding studies.. IUBMB Life, Wiley, 2014, 66 (7), pp.449-52. ⟨10.1002/iub.1287⟩. ⟨pasteur-01181367⟩

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