The periplasmic protein TolB as a potential drug target in Pseudomonas aeruginosa. - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2014

The periplasmic protein TolB as a potential drug target in Pseudomonas aeruginosa.

(1) , (1) , (2) , (2) , (2) , (3, 1)
1
2
3

Abstract

The Gram-negative bacterium Pseudomonas aeruginosa is one of the most dreaded pathogens in the hospital setting, and represents a prototype of multi-drug resistant "superbug" for which effective therapeutic options are very limited. The identification and characterization of new cellular functions that are essential for P. aeruginosa viability and/or virulence could drive the development of anti-Pseudomonas compounds with novel mechanisms of action. In this study we investigated whether TolB, the periplasmic component of the Tol-Pal trans-envelope protein complex of Gram-negative bacteria, represents a potential drug target in P. aeruginosa. By combining conditional mutagenesis with the analysis of specific pathogenicity-related phenotypes, we demonstrated that TolB is essential for P. aeruginosa growth, both in laboratory and clinical strains, and that TolB-depleted P. aeruginosa cells are strongly defective in cell-envelope integrity, resistance to human serum and several antibiotics, as well as in the ability to cause infection and persist in an insect model of P. aeruginosa infection. The essentiality of TolB for P. aeruginosa growth, resistance and pathogenicity highlights the potential of TolB as a novel molecular target for anti-P. aeruginosa drug discovery.
Fichier principal
Vignette du fichier
pone.0103784.pdf (657.25 Ko) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-01181371 , version 1 (30-07-2015)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Alessandra Lo Sciuto, Regina Fernández-Piñar, Lucia Bertuccini, Francesca Iosi, Fabiana Superti, et al.. The periplasmic protein TolB as a potential drug target in Pseudomonas aeruginosa.. PLoS ONE, 2014, 9 (8), pp.e103784. ⟨10.1371/journal.pone.0103784⟩. ⟨pasteur-01181371⟩

Collections

RIIP RIIP_FCB
82 View
207 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More