DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1.

Abstract : The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of the light-mediated seed germination process. DAG1 acts downstream PHYTOCHROME INTERACTING FACTOR3-LIKE 5 (PIL5), the master repressor, and negatively regulates gibberellin biosynthesis by directly repressing the biosynthetic gene AtGA3ox1. The Dof protein DOF AFFECTING GERMINATION (DAG2) shares a high degree of aminoacidic identity with DAG1. While DAG1 inactivation considerably increases the germination capability of seeds, the dag2 mutant has seeds with a germination potential substantially lower than the wild-type, indicating that these factors may play opposite roles in seed germination. We show here that DAG2 expression is positively regulated by environmental factors triggering germination, whereas its expression is repressed by PIL5 and DAG1; by Chromatin Immuno Precipitation (ChIP) analysis we prove that DAG1 directly regulates DAG2. In addition, we show that Red light significantly reduces germination of dag2 mutant seeds. In agreement with the seed germination phenotype of the dag2 mutant previously published, the present data prove that DAG2 is a positive regulator of the light-mediated seed germination process, and particularly reveal that this protein plays its main role downstream of PIL5 and DAG1 in the phytochrome B (phyB)-mediated pathway.
Type de document :
Article dans une revue
BMC Plant Biology, BioMed Central, 2015, 15, pp.72. 〈10.1186/s12870-015-0453-1〉
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

https://hal-riip.archives-ouvertes.fr/pasteur-01182789
Contributeur : Istituto Pasteur Fondazione Cenci Bolognetti <>
Soumis le : lundi 3 août 2015 - 15:29:46
Dernière modification le : lundi 8 octobre 2018 - 17:44:07
Document(s) archivé(s) le : mercredi 4 novembre 2015 - 10:23:28

Fichier

12870_2015_Article_453.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Silvia Santopolo, Alessandra Boccaccini, Riccardo Lorrai, Veronica Ruta, Davide Capauto, et al.. DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1.. BMC Plant Biology, BioMed Central, 2015, 15, pp.72. 〈10.1186/s12870-015-0453-1〉. 〈pasteur-01182789〉

Partager

Métriques

Consultations de la notice

135

Téléchargements de fichiers

99