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Journal articles
Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes.
T Colasanti
1, 2
M Vomero
1
C Alessandri
3
C Barbati
1, 4
A Maselli
1
C Camperio
5
F Conti
3
A Tinari
6
C Carlo-Stella
7, 8
L Tuosto
5
D Benincasa
9, 10
G Valesini
3
W Malorni
4, 11, *
M Pierdominici
1
E Ortona
1, 4
*
Corresponding author
3
Department of Internal Medicine, Lupus Clinic (Italy)
- Sapienza University [Rome] (Università degli Studi di Roma "La Sapienza" Piazzale Aldo Moro 5, 00185 Roma - Italy)
5
Department of Biology and Biotechnology "Charles Darwin" (Italy)
- Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti (Université de Rome La Sapienza 5 Place Aldo Moro 00185 Rome - Italy)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
- Università degli Studi di Roma "La Sapienza" [Rome] (Piazzale Aldo Moro 5, 00185 Roma - Italy)
7
Department of Oncology and Hematology, Humanitas Cancer Center (Italy)
- IRCCS Milan - Istituto Clinico Humanitas [Milan] (Via Alessandro Manzoni, 56, 20089 Rozzano MI - Italy)
- Hunimed - Humanitas University [Milan] (Via Rita Levi Montalcini, 4, 20090 Pieve Emanuele MI - Italy)
8
Department of Medical Biotechnology and Translational Medicine (Milan 20129 - Italy)
- CNR Neuroscience Institute (Milan 20129 - Italy)
- University of Milan (Via Festa del Perdono 7 20122 Milano - Italy)
9
Laboratory of Clinical and Behavioral Neurology (Italy)
- IRCCS - Fondazione Santa Lucia (Rome - Italy)
10
Movement Disorder Unit, Sant’Andrea Hospital (Italy)
- Università degli Studi di Roma "La Sapienza" [Rome] (Piazzale Aldo Moro 5, 00185 Roma - Italy)
11
Department of Therapeutic Research and Medicine Evaluation (Italy)
- Istituto Superiore di Sanità (Viale Regina Elena 299, 00161 Rome - Italy)
Abstract : It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of α-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the α-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of α-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of α-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of α-synuclein aggregates. These results suggest that α-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice.
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Journal articles
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https://hal-riip.archives-ouvertes.fr/pasteur-01197320
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Friday, September 11, 2015 - 2:47:29 PM
Last modification on : Wednesday, October 28, 2020 - 3:20:08 PM
Long-term archiving on: : Tuesday, December 29, 2015 - 12:39:38 AM
Contributor : Istituto Pasteur Fondazione Cenci Bolognetti <>
Submitted on : Friday, September 11, 2015 - 2:47:29 PM
Last modification on : Wednesday, October 28, 2020 - 3:20:08 PM
Long-term archiving on: : Tuesday, December 29, 2015 - 12:39:38 AM
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Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License
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- HAL Id : pasteur-01197320, version 1
- PUBMEDCENTRAL : PMC4047919
- DOI : 10.1038/cddis.2014.211
- PUBMED : 24874737
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T Colasanti, M Vomero, C Alessandri, C Barbati, A Maselli, et al.. Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes.. Cell Death and Disease, Nature Publishing Group, 2014, 5, pp.e1265. ⟨10.1038/cddis.2014.211⟩. ⟨pasteur-01197320⟩
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