SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation. - Archive ouverte HAL Access content directly
Journal Articles Cell Death and Disease Year : 2014

SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation.

(1) , (1) , (2) , (3) , (4) , (4) , (3) , (5, 2) , (1) , (1)
1
2
3
4
5

Abstract

A disintegrin and metalloproteinase 10 (ADAM10) is the major α-secretase that catalyzes the amyloid precursor protein (APP) ectodomain shedding in the brain and prevents amyloid formation. Its activity depends on correct intracellular trafficking and on synaptic membrane insertion. Here, we describe that in hippocampal neurons the synapse-associated protein-97 (SAP97), an excitatory synapse scaffolding element, governs ADAM10 trafficking from dendritic Golgi outposts to synaptic membranes. This process is mediated by a previously uncharacterized protein kinase C phosphosite in SAP97 SRC homology 3 domain that modulates SAP97 association with ADAM10. Such mechanism is essential for ADAM10 trafficking from the Golgi outposts to the synapse, but does not affect ADAM10 transport from the endoplasmic reticulum. Notably, this process is altered in Alzheimer's disease brains. These results help in understanding the mechanism responsible for the modulation of ADAM10 intracellular path, and can constitute an innovative therapeutic strategy to finely tune ADAM10 shedding activity towards APP.
Fichier principal
Vignette du fichier
cddis2014492a.pdf (4.68 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

pasteur-01203115 , version 1 (22-09-2015)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Claudia Saraceno, Elena Marcello, Daniele Di Marino, Barbara Borroni, Sylvie Claeysen, et al.. SAP97-mediated ADAM10 trafficking from Golgi outposts depends on PKC phosphorylation.. Cell Death and Disease, 2014, 5, pp.e1547. ⟨10.1038/cddis.2014.492⟩. ⟨pasteur-01203115⟩
233 View
160 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More