Detection and Isolation of Plant-Associated Bacteria Scavenging Atmospheric Molecular Hydrogen.

Abstract : High-affinity H2 -oxidizing bacteria possessing group 5 [NiFe]-hydrogenase genes are important contributors to atmospheric hydrogen (H2 ) uptake in soil environments. Although previous studies reported the occurrence of a significant H2 uptake activity in vegetation, there has been no report on the identification and diversity of the responsible microorganisms. Here, we show the existence of plant-associated bacteria with the ability to consume atmospheric H2 that may be a potential energy source required for their persistence in plants. Detection of the gene hhyL - encoding the large subunit of group 5 [NiFe]-hydrogenase - in plant tissues showed that plant-associated high-affinity H2 -oxidizing bacteria are widely distributed in herbaceous plants. Among a collection of 145 endophytic isolates, 7 Streptomyces strains were shown to possess hhyL gene and exhibit high- or intermediate-affinity H2 uptake activity. Inoculation of Arabidopsis thaliana (thale cress) and Oryza sativa (rice) seedlings with selected isolates resulted in an internalization of the bacteria in plant tissues. H2 uptake activity per bacterial cells was comparable between plant and soil, demonstrating that both environments are favorable for the H2 uptake activity of streptomycetes. This study first demonstrated the occurrence of plant-associated high-affinity H2 -oxidizing bacteria and proposed their potential contribution as a sink for atmospheric H2 .
Document type :
Journal articles
Complete list of metadatas

Cited literature [57 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01351207
Contributor : Michel Courcelles <>
Submitted on : Tuesday, August 2, 2016 - 10:11:28 PM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM
Long-term archiving on : Tuesday, November 8, 2016 - 9:49:41 PM

File

Kanno_et_al-2016-Environmental...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Manabu Kanno, Philippe Constant, Hideyuki Tamaki, Yoichi Kamagata. Detection and Isolation of Plant-Associated Bacteria Scavenging Atmospheric Molecular Hydrogen.. Environmental Microbiology, Wiley-Blackwell, 2016, ⟨10.1111/1462-2920.13162 ⟩. ⟨pasteur-01351207⟩

Share

Metrics

Record views

60

Files downloads

128