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Automated design of hammerhead ribozymes and validation by targeting the PABPN1 gene transcript.

Abstract : We present a new publicly accessible web-service, RiboSoft, which implements a comprehensive hammerhead ribozyme design procedure. It accepts as input a target sequence (and some design parameters) then generates a set of ranked hammerhead ribozymes, which target the input sequence. This paper describes the implemented procedure, which takes into consideration multiple objectives leading to a multi-objective ranking of the computer-generated ribozymes. Many ribozymes were assayed and validated, including four ribozymes targeting the transcript of a disease-causing gene (a mutant version of PABPN1). These four ribozymes were successfully tested in vitro and in vivo, for their ability to cleave the targeted transcript. The wet-lab positive results of the test are presented here demonstrating the real-world potential of both hammerhead ribozymes and RiboSoft. RiboSoft is freely available at the website
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Contributor : Michel Courcelles Connect in order to contact the contributor
Submitted on : Tuesday, August 2, 2016 - 10:29:05 PM
Last modification on : Wednesday, December 19, 2018 - 11:56:03 AM
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Nucl. Acids Res.-2016-Kharma-e...
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Nawwaf Kharma, Luc Varin, Aida Abu-Baker, Jonathan Ouellet, Sabrine Najeh, et al.. Automated design of hammerhead ribozymes and validation by targeting the PABPN1 gene transcript.. Nucleic Acids Research, Oxford University Press, 2016, 44 (4), pp.e39. ⟨10.1093/nar/gkv1111⟩. ⟨pasteur-01351212⟩



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