Regulation of viral gene expression by the herpes simplex virus 1UL24 protein (HSV-1UL24 inhibits accumulation of viral transcripts).

Carolina Sanabria-Solano 1 Carmen Elena Gonzalez 1 Nicolas Richerioux 1 Luc Bertrand 1 Slimane Dridi 1 Anthony Griffiths 2 Yves Langelier 3 Angela Pearson 1
1 INRS-IAF - Institut Armand Frappier
2 Texas Biomedical Research Institute
3 CRCHUM - Centre de recherche du Chum [Montréal]
Abstract : UL24 is conserved among all Herpesviridae. In herpes simplex virus 1 (HSV-1), UL24 mutations lead to reduced viral titers both in cell culture and in vivo, and reduced pathogenicity. The human cytomegalovirus ortholog of UL24 has a gene regulatory function; however, it is not known whether other UL24 orthologs also affect gene expression. We discovered that in co-transfection experiments, expression of UL24 correlated with a reduction in the expression of several viral proteins and transcripts. Substitution mutations targeting conserved residues in UL24 impaired this function. Reduced transcript levels did not appear attributable to changes in mRNA stability. The UL24 ortholog of Herpes B virus exhibited a similar activity. An HSV-1 mutant that does not express UL24 produced more viral R1 and R2 transcripts than the wild type or rescue virus relative to the amount of viral DNA. These results reveal a new role for HSV-1UL24 in regulating viral mRNA accumulation.
Keywords : Herpes simplex virus 1 UL24 Viral gene regulation
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Journal articles
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Contributor : Michel Courcelles <>
Submitted on : Wednesday, August 3, 2016 - 10:24:54 PM
Last modification on : Friday, September 20, 2019 - 2:04:05 PM

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Carolina Sanabria-Solano, Carmen Elena Gonzalez, Nicolas Richerioux, Luc Bertrand, Slimane Dridi, et al.. Regulation of viral gene expression by the herpes simplex virus 1UL24 protein (HSV-1UL24 inhibits accumulation of viral transcripts).. Virology, Elsevier, 2016, 495, pp.148-60. ⟨10.1016/j.virol.2016.05.006 ⟩. ⟨pasteur-01351545⟩

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