Human eosinophils are direct targets to nanoparticles: Zinc oxide nanoparticles (ZnO) delay apoptosis and increase the production of the pro-inflammatory cytokines IL-1β and IL-8.

Abstract : Zinc oxide NPs (ZnO) have been recently proposed as novel candidates for the treatment of allergic inflammatory diseases. Paradoxically, recent data suggested that ZnO could cause eosinophilic airway inflammation in rodents. Despite the above observations, there are currently no studies reporting direct interaction between a given NP and human eosinophils themselves. In this study, freshly isolated human eosinophils were incubated with ZnO and several cellular functions were studied. We found that ZnO delay human eosinophil apoptosis, partially by inhibiting caspases and by preventing caspase-4 and Bcl-xL degradation. ZnO do not induce production of reactive oxygen species but increase de novo protein synthesis. In addition, ZnO were found to increase the production of the proinflammatory IL-1β and IL-8 cytokines. Using a pharmacological approach, we demonstrated that inhibition of caspase-1 reversed the ability of ZnO to induce IL-1β and IL-8 production, whereas inhibition of caspase-4 only reversed that of IL-8. Our results indicate the necessity of conducting studies to determine the potential of using NP as nanotherapies, particularly in diseases in which eosinophils may be involved. We conclude that, indeed, human eosinophils represent potential new direct targets to NPs, ZnO in the present case.
Type de document :
Article dans une revue
Toxicology Letters, Elsevier, 2016, 259, pp.11-20. 〈10.1016/j.toxlet.2016.07.020 〉
Liste complète des métadonnées

https://hal-riip.archives-ouvertes.fr/pasteur-01351548
Contributeur : Michel Courcelles <>
Soumis le : mercredi 3 août 2016 - 22:38:38
Dernière modification le : lundi 5 février 2018 - 15:22:10

Identifiants

Collections

Citation

Luis Rafael Silva, Denis Girard. Human eosinophils are direct targets to nanoparticles: Zinc oxide nanoparticles (ZnO) delay apoptosis and increase the production of the pro-inflammatory cytokines IL-1β and IL-8.. Toxicology Letters, Elsevier, 2016, 259, pp.11-20. 〈10.1016/j.toxlet.2016.07.020 〉. 〈pasteur-01351548〉

Partager

Métriques

Consultations de la notice

45