Skip to Main content Skip to Navigation
Journal articles

Surveying the endomicrobiome and ectomicrobiome of bark beetles: The case of Dendroctonus simplex.

Abstract : Many bark beetles belonging to the Dendroctonus genus carry bacterial and fungal microbiota, forming a symbiotic complex that helps the insect to colonize the subcortical environment of the host tree. However, the biodiversity of those bacteria at the surface of the cuticle or inside the body parts of bark beetles is not well established. The aim of this study was to characterize the bacterial microbiome associated with the eastern larch beetle, Dendroctonus simplex, using bacterial 16S rRNA gene pyrosequencing. The ecto- and endomicrobiome and the subcortical galleries were investigated. Several bacterial genera were identified, among which Pseudomonas, Serratia and Yersinia are associated with the surface of the beetle cuticle, and genera belonging to Enterobacteriaceae and Gammaproteobacteria with the interior of the insect body. The index of dissimilarity indicates that the bacterial microbiome associated with each environment constitutes exclusive groups. These results suggest the presence of distinct bacterial microbiota on the surface of the cuticle and the interior of D. simplex body. Additionally, the bacterial diversity identified in the galleries is substantially different from the ectomicrobiome, which could indicate a selection by the insect. This study reports for the first time the identification of the eastern larch beetle microbiome.
Keywords : Entomology Microbiome
Complete list of metadatas

Cited literature [50 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-01351928
Contributor : Michel Courcelles <>
Submitted on : Thursday, August 4, 2016 - 10:51:08 PM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM

File

srep17190.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Audrey-Anne Durand, Amélie Bergeron, Philippe Constant, Jean-Philippe Buffet, Eric Déziel, et al.. Surveying the endomicrobiome and ectomicrobiome of bark beetles: The case of Dendroctonus simplex.. Scientific Reports, Nature Publishing Group, 2015, 5, pp.17190. ⟨10.1038/srep17190⟩. ⟨pasteur-01351928⟩

Share

Metrics

Record views

243

Files downloads

246