Fetal Hemoglobin in Tunisian Sickle Cell Disease Patient: Relationship with Polymorphic Sequences Cis to the β-Globin Gene

Imen Moumni 1, * Maha Ben Mustapha 1 Ikbel Ben Mansour 1 Amine Zoraï 1 Kaïs Douzi 1 Sarah Sassi Dorra Chaouachi 1 Fethi Mellouli 2 Mohamed Bejaoui 2 Salem Abbes 1
* Auteur correspondant
1 LR11IPT07 - Laboratoire d'hématologie moléculaire et cellulaire
2 Department of Peadiatric Immuno-Haematology
Abstract : Fetal hemoglobin (HbF) plays a dominant role in ameliorating morbidity and mortality of hemoglobinopathies. We evaluated the effects of polymorphic markers within the beta-globin gene cluster to identify the genetic mechanics that influence HbF on Tunisian sickling patients (n = 242). Haplotype analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the framework polymorphism was established by PCR-sequencing, four independent regions of interest were identified: the 5' region of beta-LCR-HS2 site, the intervening sequence II (IVSII) region of two fetal (G gamma and A gamma) genes and the 5' region of beta-globin gene. The correlation of these various Haplotypes and SNPs with HbF expression and clinical data was studied. Our data showed that among the various polymorphic markers analyzed, only the sequence (AT)xN12(AT)y in LCR HS2 region was significantly associated (p < 0.05) with increased HbF levels, suggesting that the beta-globin gene cluster exerts a significant effect on HbF in sickle cell patients. This study can improve understanding of the physiopathology of the disease and aid to increase our ability to predict clinical severity.
Keywords : Microsatellite repeats Fetal hemoglobin (HbF) Sickle cell anemia
Type de document :
Article dans une revue
Indian Journal of Hematology and Blood Transfusion, Bombay: Indian Society of Hematology & Blood Transfusion., 2016, 32 (1), pp.114 - 119. 〈10.1007/s12288-015-0504-7〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01374975
Contributeur : Institut Pasteur Tunis <>
Soumis le : mardi 6 décembre 2016 - 12:36:21
Dernière modification le : lundi 5 février 2018 - 15:22:12

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Imen Moumni, Maha Ben Mustapha, Ikbel Ben Mansour, Amine Zoraï, Kaïs Douzi, et al.. Fetal Hemoglobin in Tunisian Sickle Cell Disease Patient: Relationship with Polymorphic Sequences Cis to the β-Globin Gene. Indian Journal of Hematology and Blood Transfusion, Bombay: Indian Society of Hematology & Blood Transfusion., 2016, 32 (1), pp.114 - 119. 〈10.1007/s12288-015-0504-7〉. 〈pasteur-01374975〉

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