In vitro probiotic profiling of novel Enterococcus faecium and Leuconostoc mesenteroides from Tunisian freshwater fishes

Jorge Barros-Velazquez 1 Balkiss Bouhaouala-Zahar 2, 3 Rim El-Jeni 3, 4 Monia El Bour 4 Pilar Calo-Mata 1 Karola Boehme 1 Inmaculada C. Fernandez-No 1
1 Dept Analyt Chem Nutr & Food Sci
2 Medical School of Tunis, University of Tunis El Manar, El Manar, Tunisia
3 LR11IPT08 - Laboratoire des Venins et Biomolécules Thérapeutiques
4 Institut National des Sciences et Technologies de la Mer [Salammbô]
Abstract : Novel lactic acid bacteria isolated from different organs of freshwater fish were examined for their potential application as probiotics in raw and processed foods. Four isolates of Enterococcus faecium and Leuconostoc mesenteroides were identified at the molecular level by 16S rRNA sequencing and random amplification of polymorphic DNA - polymerase chain reaction, and their antimicrobial activity against a panel of pathogens and food-poisoning bacteria was investigated. The whole bacteriocins of the 4 isolates were characterized by enterobacterial repetitive intergenic consensus sequences in PCR. The isolates exhibited high inhibitory activities against food-borne pathogens and spoilage microbial species and have significant probiotic profiles, since they survived at pH 3.0 and in the presence of bile salts, pancreatin, and pepsin, without any detectable hemolytic activity. Further, moderate heat resistance, adhesion ability to steel surfaces, and sensitivity to clinically relevant antimicrobial agents were revealed for all the isolates. These results highlight the specific probiotic properties of the strains and give evidence for potential application in minimally processed foods subjected to moderate heat processing.
Keywords : processed foods probiotics bacteriocins freshwater fish Enterococcus Leuconostoc food biopreservation enterocins
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Article dans une revue
Canadian Journal of Microbiology, NRC Research Press, 2016, 62 (1), pp.60-71. 〈10.1139/cjm-2015-0481〉
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https://hal-riip.archives-ouvertes.fr/pasteur-01374979
Contributeur : Institut Pasteur Tunis <>
Soumis le : mercredi 21 décembre 2016 - 15:23:42
Dernière modification le : lundi 5 février 2018 - 15:22:13

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Jorge Barros-Velazquez, Balkiss Bouhaouala-Zahar, Rim El-Jeni, Monia El Bour, Pilar Calo-Mata, et al.. In vitro probiotic profiling of novel Enterococcus faecium and Leuconostoc mesenteroides from Tunisian freshwater fishes. Canadian Journal of Microbiology, NRC Research Press, 2016, 62 (1), pp.60-71. 〈10.1139/cjm-2015-0481〉. 〈pasteur-01374979〉

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