Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Molecular diagnosis of Rickettsia infection in patients from Tunisia

Abstract : Diagnosis of rickettsioses had largely benefited from the development of molecular techniques. Unfortunately, in Tunisia, despite the large number of rickettsia( cases registered every year, the Rickettsia species remain unidentified. In this study, we aimed to detect the Rickettsia species in clinical samples using molecular tests. A study was established to analyze skin biopsies, cutaneous swabs, and cerebrospinal fluid samples taken from clinically suspected patients to have rickettsial infection. Two molecular techniques were used to detect Rickettsia DNA: quantitative real time PCR (qPCR) and reverse line blot test (RLB). An analysis of the RLB hybridization assay results revealed the presence of Rickettsia DNA in skin biopsies (40.6%) and swabs (46.7%). Rickettsia conorii was the most prevalent identified species among tested samples. Other species of interest include Rickettsia typhi and Rickettsia massiliae. Using qPCR positivity rates in skin biopsies was 63.7% against 80% in swabs. R. conorii was the most frequently detected species, followed by R typhi. The agreement between the two techniques was 68.6% (kappa = 0.33). Molecular tests, especially using specific probes qPCR, allow for a rapid, better and confident diagnosis in clinical practice. They improve the survey of Mediterranean spotted fever which is considered to be the most important rickettsial infection in humans in Tunisia. (C) 2016 Elsevier GmbH. All rights reserved.
Complete list of metadata
Contributor : Institut Pasteur Tunis Connect in order to contact the contributor
Submitted on : Wednesday, December 28, 2016 - 9:42:48 AM
Last modification on : Wednesday, September 8, 2021 - 1:50:26 PM




Fatma Khrouf, Hanene Sellami, Emna Elleuch, Zouhour Hattab, Lamia Ammari, et al.. Molecular diagnosis of Rickettsia infection in patients from Tunisia. Ticks and Tick-borne Diseases, Elsevier, 2016, 7 (5), pp.653 - 656. ⟨10.1016/j.ttbdis.2016.02.010⟩. ⟨pasteur-01374982⟩



Record views