Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells

Abstract : Context: Within the global context of increasing cancer diseases, natural products are important in devising new drugs and providing unique ideas in cancer therapy. In Tunisian folk medicine, Pistacia lentiscus L. (Anacardiaceae) fixed oil is used for cancer treatment. Objective: This investigation studied, for the first time, the antiproliferative effect of Pistacia lentiscus fixed oil and its phenolic extract on BHK21 cancer cells. Materials and methods: Oil was extracted from fruits harvested in northwest Tunisia and the phenolic fraction was obtained by mixing with methanol. The anti-proliferative activity of the two tested substances on BHK 21 cells were investigated in vitro using trypan blue assays. Cells were treated with different concentrations of P. lentiscus oil (0.009, 0.018, 0.036, and 0.09 g/mL) and the phenolic extract (0.007, 0.014, 0.03, and 0.07 g/mL) for 24, 48, and 72 h. Results: The inhibitory effect of Pistacia lentiscus fixed oil increases with the increase in dose. The IC50 value was estimated at 0.029 g/mL. The percentage of cell viability was 42.46 ± 3.4% at a dose of 0.09 g/mL and was significantly lower than that of the untreated control (96.24 ± 2.5%, p<0.01). The phenolic extract demonstrated a dose- and time-dependent inhibitory effect on BHK21 cell growth. After 48 h of incubation, the IC50 value was estimated at 0.15 g/mL. Discussion and conclusion: The results demonstrated the potential of Pistacia lentiscus fixed oil in treating cancer, as it is used in traditional medicine.
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Pharmaceutical Biology, Taylor & Francis, 2016, 54 (5), pp.747-751
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https://hal-riip.archives-ouvertes.fr/pasteur-01374998
Contributeur : Institut Pasteur Tunis <>
Soumis le : jeudi 29 décembre 2016 - 10:25:57
Dernière modification le : jeudi 20 septembre 2018 - 14:30:02

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  • HAL Id : pasteur-01374998, version 1

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Faten Mezni, Sarra Shili, Nejia Ben Ali, Mohamed Larbi Khouja, Abdelhamid Khaldi, et al.. Evaluation of Pistacia lentiscus seed oil and phenolic compounds for in vitro antiproliferative effects against BHK21 cells. Pharmaceutical Biology, Taylor & Francis, 2016, 54 (5), pp.747-751. 〈pasteur-01374998〉

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