The galactolipase activity of Fusarium solani (phospho)lipase

Raida Jallouli 1 Houcemeddine Othman 2 Sawsan Amara 3 Goetz Parsiegla 3 Frédéric Carriere 3 Najet Srairi-Abid 2 Youssef Gargouri 1 Sofiane Bezzine 1, *
* Auteur correspondant
1 Université de Sfax
2 LR11IPT08 - Laboratoire des Venins et Biomolécules Thérapeutiques
3 EIPL - Enzymologie interfaciale et de physiologie de la lipolyse
Abstract : The purified (phospho)lipase of Fusarium solani (FSL), was known to be active on both triglycerides and phospholipids. This study aimed at assessing the potential of this enzyme in hydrolyzing galactolipids. FSL was found to hydrolyze at high rates of synthetic medium chains monogalactosyldiacylglycerol (4658 +/- 146 U/mg on DiC8-MGDG) and digalactosyldiacylglycerol (3785 +/- 83 U/mg on DiC8-DGDG) and natural long chain monogalactosyldiacylglycerol extracted from leek leaves (991 +/- 85 U/mg). It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. FSL maximum activity on galactolipids was measured at pH 8. The analysis of the hydrolysis product of natural MGDG from leek showed that FSL hydrolyzes preferentially the ester bond at the sn-1 position of galactolipids. To investigate the structure-activity relationships of FSL, a 3D model of this enzyme was built. In silico docking of medium chains MGDG and DGDG and phospholipid in the active site of FSL reveals structural solutions which are in concordance with in vitro tests. 2015 Elsevier B.V. All rights reserved.
Keywords : Galactolipid Galactolipase Fusarium solani (phospho)lipase Leek In silico docking
Type de document :
Article dans une revue
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, Elsevier, 2015, 1851 (3), pp.282 - 289. 〈10.1016/j.bbalip.2014.12.010〉
Liste complète des métadonnées
https://hal-riip.archives-ouvertes.fr/pasteur-01375005
Contributeur : Institut Pasteur Tunis <>
Soumis le : lundi 2 janvier 2017 - 13:19:51
Dernière modification le : jeudi 20 septembre 2018 - 15:04:03

Identifiants

Collections

RIIP | UNIV-AMU | RIIP_TUNIS

Citation

Raida Jallouli, Houcemeddine Othman, Sawsan Amara, Goetz Parsiegla, Frédéric Carriere, et al.. The galactolipase activity of Fusarium solani (phospho)lipase. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, Elsevier, 2015, 1851 (3), pp.282 - 289. 〈10.1016/j.bbalip.2014.12.010〉. 〈pasteur-01375005〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

47

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD