A. Tabbabi, A. Rhim, A. Bouratbine, and K. Aoun, Authors E-mails: tabbabiahmed@ gmail.com, adel.rhaim@pasteur.rns.tn, aida.bouratbine@pasteur.rns .tn, and karim.aoun@pasteur.rns.tn. Philippe Boussès, Cécile Brengues, Didier Fontenille, and Frédéric Simard, Maladies Infectieuses et Vecteurs: E ´ cologie

A. Chadli, M. Kennou, and J. Kooli, Le paludisme en Tunisie: historique et e ´ tat actuel, Bull Soc Pathol Exot, vol.78, pp.844-851, 1985.

A. Chadli, M. Kennoun, and J. Kooli, Les campagnes d'e ´ radication du paludisme en Tunisie: historique et e ´ tat actuel, Arch Inst Pasteur Tunis, vol.63, pp.35-50, 1986.

B. Rachid, M. , B. Ammar, R. Redissi, T. et al., Gé ographie des parasitoses majeures en Tunisie, Arch Inst Pasteur Tunis, vol.61, pp.17-41, 1984.

. Anonymous, Bulletins e ´pidémiologiques de la Direction de Soins de Santé de Base-Ministère de la Santé publique, 1980.

D. Gmara, Situation actuelle du paludisme dans le monde et en Tunisie (DSSB), 2006.

A. Bouratbine, M. Chahed, K. Aoun, G. Krida, S. Ayari et al., Le paludisme d'importation en Tunisie, Bull Soc Pathol Exot, vol.91, pp.203-207, 1998.

J. Brunhes, A. Rhaim, B. Geoffroy, G. Angel, and J. Hervy, Les moustiques de l'Afrique méditerranéenne CD-ROM d'identification et d'enseignement, Edition IRD, 1999.

B. Bouchité, M. Kennou, and G. Chauvet, Ethologie et capacité vectorielle des anophèles de Tunisie dans la région de Sidi Bouzi, multigrade, 1991.

A. Bouattour, A. Rhaim, and D. Bach-hamba, Etude de la capacité vectorielle d'Anopheles labranchiae dans la région ANOPHELES MOSQUITOES IN TUNISIA de Nefza, 1993.

G. Krida, A. Bouattour, A. Rhaim, A. Kebir, and R. Jlidi, Preliminary investigation of four Anopheles larvae samples susceptibility to chlorpyrifos in Tunisia, Arch Inst Pasteur Tunis, vol.75, pp.199-203, 1998.

M. Sedaghat, Y. Linton, M. Oshaghi, H. Vatandoost, and R. Harbach, The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species, Bulletin of Entomological Research, vol.37, issue.06, pp.527-535, 2003.
DOI : 10.1016/S0001-706X(01)00080-8

G. Nicolescu, Y. Linton, A. Vladimirescu, T. Howard, and R. Harbach, Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence, Bulletin of Entomological Research, vol.13, issue.06, pp.525-535, 2004.
DOI : 10.1046/j.1365-2583.1999.00140.x

Y. Linton, L. Smith, and R. Harbach, Observations on the taxonomic status of Anopheles subalpinus Hackett and Lewis and An. melanoon Hackett, Eur Mosq Bull, vol.13, pp.8-16, 2002.

E. Patsoula, A. Samanidou-voyadjoglou, G. Spanakos, J. Kremastinou, G. Nasioulas et al., Molecular characterization of the Anopheles maculipennis complex during surveillance for the 2004 Olympic Games in Athens, Medical and Veterinary Entomology, vol.23, issue.1, pp.36-43, 2007.
DOI : 10.1016/1055-7903(92)90001-W

S. Doosti, S. Azari-hamidian, H. Vatandoost, M. Oshaghi, and M. Hosseini, Taxonomic differentiation of Anopheles sacharovi and An. maculipennis s.l. (Diptera: Culicidae) larvae by seta 2 (antepalmate hair), Acta Med Iran, vol.44, pp.21-27, 2006.

S. Doosti, H. Vatandoost, M. Oshaghi, M. Hosseini, and M. Sedaghat, Applying morphometric variation of seta 2 (antepalmate hair) among the larvae of the members of the Maculipennis subgroup (Diptera: Culicidae) in Iran, Iran J Arthropod Borne Dis, vol.1, pp.28-37, 2007.

Y. Linton, A. Samanidou-voyadjoglou, and R. Harbach, Ribosomal ITS2 sequence data for Anopheles maculipennis and An. messeae in northern Greece, with a critical assessment of previously published sequences, Insect Molecular Biology, vol.23, issue.4, pp.379-383, 2002.
DOI : 10.1093/nar/25.24.4876

D. Boccolini, D. Luca, M. Marinucci, M. Romi, and R. , Further molecular and morphological support for the formal synonymy of Anopheles subalpinus Hackett and Lewis with An. melanoon Hackett, Eur Mosq Bull, vol.16, pp.1-5, 2003.

D. Luca, M. Boccolini, D. Marinucci, M. Romi, and R. , Complex) Inferred by Molecular Analysis, Journal of Medical Entomology, vol.41, issue.4, pp.582-586, 2004.
DOI : 10.1603/0022-2585-41.4.582

N. Djadid, S. Gholizadeh, E. Tafsiri, R. Romi, M. Gordeev et al., Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran, Malaria Journal, vol.6, issue.1, p.6, 2007.
DOI : 10.1186/1475-2875-6-6

H. Kampen, Integration of Anopheles beklemishevi (Diptera: Culicidae) in a PCR assay diagnostic for palaearctic Anopheles maculipennis sibling species, Parasitology Research, vol.72, issue.2, pp.113-117, 2005.
DOI : 10.1007/s00436-005-1392-9

M. Laboudi, C. Faraj, A. Sadak, Z. Harrat, S. Boubidi et al., DNA barcodes confirm the presence of a single member of the Anopheles maculipennis group in Morocco and Algeria: An. sicaulti is conspecific with An. labranchiae, Acta Tropica, vol.118, issue.1, pp.6-13, 2011.
DOI : 10.1016/j.actatropica.2010.12.006

Y. Linton, A. Samanidou-voyadjoglou, L. Smith, and R. Harbach, New occurrence records for Anopheles maculipennis and An. messeae in northern Greece based on DNA sequence data, Eur Mosq Bull, vol.11, pp.31-36, 2001.

Y. Linton, L. Smith, and R. Harbach, Molecular confirmation of sympatric populations of Anopheles messeae and Anopheles atroparvus overwintering in Kent, southeast England, Eur Mosq Bull, vol.13, pp.8-16, 2002.

Y. Linton, L. Smith, G. Koliopoulos, A. Samanidou-voyadjoglou, A. Zounos et al., Morphological and molecular characterization of Anopheles (Anopheles) maculipennis Meigen, type species of the genus and nominotypical member of the Maculipennis Complex, Systematic Entomology, vol.23, issue.Suppl., pp.39-55, 2003.
DOI : 10.1046/j.1365-3113.2003.00198.x

Y. Linton, A. Lee, and C. Curtis, Discovery of third member of Maculipennis group in SW England, Eur Mosq Bull, vol.19, pp.5-9, 2005.

Y. Linton, L. Smith, G. Koliopoulos, A. Zounos, A. Samanidou-voyadjoglou et al., complex (Diptera: Culicidae) in Greece, Journal of Natural History, vol.77, issue.41-44, pp.2683-2699, 2007.
DOI : 10.1080/00222930701403255

M. Marinucci, R. Romi, P. Mancini, D. Luca, M. Severini et al., Phylogenetic relationships of seven palearctic members of the maculipennis complex inferred from ITS2 sequence analysis, Insect Molecular Biology, vol.11, issue.4, pp.469-480, 1999.
DOI : 10.1086/417338

M. Service, Mosquito Ecology: Field Sampling Methods, 1993.

J. Proft, W. Maier, and H. Kampen, Identification of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay, Parasitology Research, vol.85, issue.10, pp.837-843, 1999.
DOI : 10.1007/s004360050642

I. Morlais, N. Ponçon, F. Simard, A. Cohuet, and D. Fontenille, Intraspecific nucleotide variation in Anopheles gambiae: new insights into the biology of malaria vectors, Am J Trop Med Hyg, vol.71, pp.795-802, 2004.

N. Herrel, F. Amerasinghe, J. Ensink, M. Mukhtar, W. Van-der-hoek et al., Breeding of Anopheles mosquitoes in irrigated areas of South Punjab, Pakistan, Medical and Veterinary Entomology, vol.36, issue.3, pp.236-248, 2001.
DOI : 10.1016/0035-9203(80)90153-4

A. Christophe, C. N. Carlo, C. Parfait, A. Didier, F. Fré-dé-ric et al., Distribution and larval habitat characterization of Anopheles moucheti, Anopheles nili, and other malaria vectors in river networks of southern Cameroon, Acta Trop, vol.112, pp.270-276, 2009.

R. Romi, D. Boccolini, D. Luca, M. , L. Rosa et al., Identification of the sibling species of the Anopheles maculipennis complex by heteroduplex analysis, Insect Molecular Biology, vol.21, issue.7, pp.509-513, 2000.
DOI : 10.1046/j.1365-2583.1999.00140.x

M. Chahed, A. Bouratbine, G. Krida, B. Hamida, and A. , Receptivity of Tunisia to malaria after its eradication: analysis of the situation for adequacy of the surveillance, Bull Soc Pathol Exot, vol.94, pp.271-276, 2001.

J. Gaud, Notes biogé ographiques sur les culicidé s au Maroc, Arch Inst Pasteur Maroc, vol.4, pp.443-490, 1953.

Y. Guy, Les Anopheles du Maroc, Mem Soc Sci Nat Phys Zool, vol.7, p.235, 1959.

Y. Guy, Bilan e ´ pidé miologique du paludisme au Maroc (donné es recueillies entre, Ann Parasitol Hum Comp, vol.38, pp.823-857, 1960.

N. Benmansour, M. Laaziri, and B. Mouki, Note sur la faune anophé lienne du Maroc, Bull Inst Hyg (Maroc), vol.52, pp.103-112, 1972.

C. Faraj, E. Adlaoui, N. Saaf, R. Romi, D. Boccolini et al., Note sur le complexe Anopheles maculipennis au Maroc, Bull Soc Pathol Exot, vol.97, pp.293-294, 2004.

G. Senevet and L. Andarelli, Les Anopheles d'Afrique du Nord et du Bassin Mé dité rrané en, Encycl Entomol, vol.33, pp.1-280, 1956.

G. Senevet and L. Andarelli, Contribution a ` l'e ´ tude de la biologie des Anopheles algé riens, Arch Inst Pasteur Alger, vol.39, pp.393-400, 1961.

P. Constantinescu and E. Negulici, The experimental transmission of Plasmodium malariae to Anopheles labranchiae atroparvus, Transactions of the Royal Society of Tropical Medicine and Hygiene, vol.61, issue.2, pp.182-188, 1967.
DOI : 10.1016/0035-9203(67)90155-1

C. Ramsdale and M. Coluzzi, Studies on the infectivity of tropical African strains of Plasmodium falciparum to some southern European vectors of malaria, Parassitologia, vol.17, pp.39-48, 1975.

J. De-zulueta, C. Ramsdale, and M. Coluzzi, Receptivity to malaria in Europe, Bull World Health Organ, vol.52, pp.109-111, 1975.

J. Sautet and R. Quilici, A propos de quelques cas de paludisme autochtone contracté s en France pendant l'e ´ té, Presse Med, vol.79, p.524, 1971.

A. Zahar, Vector Bionomics in the Epidemiology and Control of Malaria. Part II: The WHO European Region and the WHO Eastern Mediterranean Region, 1987.

M. Baldari, A. Tamburro, G. Sabatinelli, R. Romi, C. Severini et al., Malaria in Maremma, Italy, The Lancet, vol.351, issue.9111, pp.1246-1248, 1998.
DOI : 10.1016/S0140-6736(97)10312-9

C. Toty, H. Barré, L. Goff, G. Larget-thié-ry, I. Rahola et al., Malaria risk in Corsica, former hot spot of malaria in France, Malaria Journal, vol.9, issue.1, p.231, 2010.
DOI : 10.1186/1475-2875-9-231

URL : https://hal.archives-ouvertes.fr/pasteur-00670626

R. Romi, D. Boccolini, R. Vallorani, F. Severini, L. Toma et al., Assessment of the risk of malaria re-introduction in the Maremma plain (Central Italy) using a multi-factorial approach, Malaria Journal, vol.11, issue.1, p.98, 2012.
DOI : 10.1186/1475-2875-9-231

URL : https://hal.archives-ouvertes.fr/pasteur-00698544

G. Houel and F. Donadille, Vingt ans de lutte antipaludique au Maroc, Bull Inst Hyg (Maroc), vol.13, pp.1-51, 1953.

A. Zahar, Review of the ecology of malaria vectors in the WHO Eastern Mediterranean Region, Bull World Health Org, vol.50, pp.427-440, 1974.

M. Kenawy, J. Beier, C. Asiago, S. Said, and C. Roberts, Interpretation of Low-Level Plasmodium Infection Rates Determined by Elisa for Anophelines (Diptera: Culicidae) from Egyptian Oases, Journal of Medical Entomology, vol.27, issue.4, pp.681-685, 1990.
DOI : 10.1093/jmedent/27.4.681

F. Peus, Die Fiebermü cken des Mittelmeergebietes, 1942.

P. Russell, L. Rozeboom, and A. Stone, Keys to the Anopheline Mosquitoes of the World, 1943.

N. Becker, D. Petric, M. Zgomba, C. Boase, M. Madon et al., Mosquitoes and Their Control, 2010.