Association study of mitochondrial DNA polymorphisms with type 2 diabetes in Tunisian population

Sana Hsouna 1 Nizar Ben Halim 2 Khaled Lasram 2 Imen Arfa 2, 3 Henda Jamoussi 2, 4 Sonia Bahri 5, 3 Slim Ben Ammar 3, 5 Najoua Miladi Abdelmajid Abid 2, 3 Sonia Abdelhak 2 Rym Kefi 2
1 Child Neurological Diseases Unit
2 LR11IPT05 - Laboratoire de Génomique Biomédicale et Oncogénétique - Biomedical Genomics and Oncogenetics Laboratory
3 UTM - Université Tunis El Manar
4 National Institute of Nutrition and Food Technology, Department of External Consultation, 11 rue Jebel Lakhdar, Bab Saadoun, 1007 Tunis, Tunisia.
5 Laboratoire central de biologie médicale, Institut Pasteur de Tunis
Abstract : Mitochondrial DNA (mtDNA) variation may play an important role in the pathogenesis of type 2 diabetes (T2Ds). In this study, we aimed to explore whether mtDNA variants contribute to the susceptibility to T2Ds in a Tunisian population. The hypervariable region 1 (HVS1) of the mtDNA of 64 T2Ds patients and 77 healthy controls was amplified and sequenced. Statistical analysis was performed using the STATA program. Analysis of the total screened variants (N = 88) from the HVS1 region showed no significant difference in the distribution of all polymorphisms between T2Ds and controls, except for the variant G16390A which was more frequent in T2Ds (15.9%) than in controls (5.4%) (p = 0.04). The association of G16390A was not detected after multivariate regression analysis. Similarly, analysis of the distribution of mitochondrial haplogroups within our dataset showed 18 distinct major haplogroups with no significant difference between T2Ds and controls. Except, the weakly association found for the G16390A variant, our results showed that none of the tested polymorphisms from the HVS1 region have a major role in T2Ds pathogenesis in the studied Tunisian population even when taking into account the population stratification.
Keywords : Hypervariable region 1 mitochondrial DNA North Africa Tunisia type 2 diabetes
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Journal articles
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Submitted on : Sunday, October 2, 2016 - 8:02:02 PM
Last modification on : Monday, October 8, 2018 - 5:44:08 PM

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Sana Hsouna, Nizar Ben Halim, Khaled Lasram, Imen Arfa, Henda Jamoussi, et al.. Association study of mitochondrial DNA polymorphisms with type 2 diabetes in Tunisian population. Mitochondrial DNA, 2015, 26 (3), pp.367-372. ⟨10.3109/19401736.2013.836508⟩. ⟨pasteur-01375027⟩

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