Hepatoprotective effect of carob against acute ethanol-induced oxidative stress in rat

Abdelaziz Souli 1, 2 Hichem Sebai 3, 2, * Latifa Chehimi 3 Kais Rtibi 1, 2 Haifa Tounsi 4 Samir Boubaker 4 Mohsen Sakly 3 Jamel El-Benna 5 Mohamed Amri 1
* Auteur correspondant
1 Faculté des Sciences de Tunis
2 Laboratoire de Nutrition et Physiologie Animale
3 Faculté des Sciences de Bizerte
4 Laboratoire d’Anatomie Pathologique Humaine et Expérimentale
5 INSERM, U773, Biomedical Research Center Bichat Beaujon
Abstract : The present study was undertaken to determine whether subacute treatment with aqueous extract of carob (Ceratonia siliqua L.) pods (AECPs) protects against ethanol (EtOH)-induced oxidative stress in rat liver. Animals were divided into four groups: control, carob, EtOH and EtOH+carob. Wistar rats were intraperitoneally pretreated with AECP (600mg/kg body weight (bw)) during 7days and intoxicated for 6h by acute oral administration of EtOH (6g/kg bw) 24h after the last injection. We found that acute administration of EtOH leads to hepatotoxicity as monitored by the increase in the levels of hepatic marker aspartate aminotransferase and alanine aminotransferase as well as hepatic tissue injury. EtOH also increased the formation of malondialdehyde in the liver, indicating an increase in lipid peroxidation and depletion of antioxidant enzyme activities as superoxide dismutase, catalase and glutathione peroxidase. Subacute carob pretreatment prevented all the alterations induced by EtOH and returned their levels to near normal. Importantly, we showed that acute alcohol increased hepatic and plasmatic hydrogen peroxide and free iron levels. The carob pretreatment reversed EtOH effects to near control levels. These data suggest that carob could have a beneficial effect in inhibiting the oxidative damage induced by acute EtOH administration and that its mode of action may involve an opposite effect on plasma and tissue-free iron accumulation. Indeed, carob can be offered as a food additive to protect against EtOH-induced oxidative damage.
Keywords : iron EtOH Carob rat liver oxidative stress EtOH iron liver oxidative stress rat
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Article dans une revue
Toxicology and Industrial Health, SAGE Publications, 2015, 31 (9), pp.802-810. 〈10.1177/0748233713475506〉
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Contributeur : Institut Pasteur Tunis <>
Soumis le : jeudi 7 septembre 2017 - 11:17:47
Dernière modification le : mercredi 18 octobre 2017 - 01:04:50

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Abdelaziz Souli, Hichem Sebai, Latifa Chehimi, Kais Rtibi, Haifa Tounsi, et al.. Hepatoprotective effect of carob against acute ethanol-induced oxidative stress in rat. Toxicology and Industrial Health, SAGE Publications, 2015, 31 (9), pp.802-810. 〈10.1177/0748233713475506〉. 〈pasteur-01375042〉

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