Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

C. Cassagne; F. Pratlong; F Jeddi; R Benikhlef; K Aoun; A-C Normand; F Faraut; P Bastien; R Piarroux

doi:10.1111/1469-0691.12387

Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

C. Cassagne ^{1, *} F. Pratlong ^{2, 3} F Jeddi ¹ R Benikhlef ⁴ K Aoun ⁵ A-C Normand ¹ F Faraut ¹ P Bastien ² R Piarroux ¹

* Auteur correspondant

1 IP-TPT - Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques

2 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle

3 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]

4 Institut Pasteur d'Algérie

5 LR11IPT06 - Laboratoire de Parasitologie Médicale, Biotechnologies et Biomolécules

Abstract : Matrix-assisted laser desorption ionization time-of-flightMALDI-TOF mass spectrometry (MS) is now widely recognized as a powerful tool with which to identify bacteria and fungi at the species level, and sometimes in a rapid and accurate manner. We report herein an approach to identify, at the species level, Leishmania promastigotes from in vitro culture. We first constructed a reference database of spectra including the main Leishmania species known to cause human leishmaniasis. Then, the performance of the reference database in identifying Leishmania promastigotes was tested on a panel of 69 isolates obtained from patients. Our approach correctly identified 66 of the 69 isolates tested at the species level with log (score) values superior to 2. Two Leishmania isolates yielded non-interpretable MALDI-TOF MS patterns, owing to low log (score) values. Only one Leishmania isolate of Leishmania peruviana was misidentified as the closely related species Leishmania braziliensis, with a log (score) of 2.399. MALDI-TOF MS is a promising approach, providing rapid and accurate identification of Leishmania from in vitro culture at the species level.

Keywords : MALDI-TOF MS mass spectra parasite Identification Leishmania

Type de document :

Article dans une revue

Clinical Microbiology and Infection, Elsevier for the European Society of Clinical Microbiology and Infectious Diseases, 2014, 20 (6), pp.551-7. 〈10.1111/1469-0691.12387〉

Domaine :

Sciences du Vivant [q-bio]

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Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

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Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

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