Skip to Main content Skip to Navigation
Journal articles

Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Abstract : Matrix-assisted laser desorption ionization time-of-flightMALDI-TOF mass spectrometry (MS) is now widely recognized as a powerful tool with which to identify bacteria and fungi at the species level, and sometimes in a rapid and accurate manner. We report herein an approach to identify, at the species level, Leishmania promastigotes from in vitro culture. We first constructed a reference database of spectra including the main Leishmania species known to cause human leishmaniasis. Then, the performance of the reference database in identifying Leishmania promastigotes was tested on a panel of 69 isolates obtained from patients. Our approach correctly identified 66 of the 69 isolates tested at the species level with log (score) values superior to 2. Two Leishmania isolates yielded non-interpretable MALDI-TOF MS patterns, owing to low log (score) values. Only one Leishmania isolate of Leishmania peruviana was misidentified as the closely related species Leishmania braziliensis, with a log (score) of 2.399. MALDI-TOF MS is a promising approach, providing rapid and accurate identification of Leishmania from in vitro culture at the species level.
Document type :
Journal articles
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-01375047
Contributor : Institut Pasteur Tunis <>
Submitted on : Sunday, October 2, 2016 - 8:02:37 PM
Last modification on : Monday, October 12, 2020 - 4:37:23 PM

Links full text

Identifiers

Citation

C. Cassagne, F. Pratlong, F Jeddi, R Benikhlef, K Aoun, et al.. Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.. Clinical Microbiology and Infection, Elsevier for the European Society of Clinical Microbiology and Infectious Diseases, 2014, 20 (6), pp.551-7. ⟨10.1111/1469-0691.12387⟩. ⟨pasteur-01375047⟩

Share

Metrics

Record views

216