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Molecular characterization and antibiotic resistance of Salmonellain children with acute gastroenteritis in Abuja, Nigeria

Résumé : In Nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including Salmonella, remains a powerful tool for effective management, surveillance, and control. Diarrheal stool samples were directly plated onto differential and selective media to isolate Salmonella. Extended-spectrum beta-lactamases were screened using the double disk diffusion technique and by PCR targeting the blaTEM and blaCTX-M genes. Pulsed-field gel electrophoresis (PFGE) was performed usingthe PulseNet Canada Laboratory protocol for molecular subtyping using the restriction enzymes XbaI and BlnI. The serotypes identified were Salmonella enterica serovar Zanzibar (n = 5), Salmonella Brancaster (n = 3), and one isolate of Salmonella Enteritidis (phage type 1). The following levels of resistance were found among the Salmonella strains: amoxicillin, five strains (55.6%); amoxicillin-clavulanic acid, two strains (22.2%); cephalexin, five strains (55.6%); and cefuroxime, five strains (55.6%). Intermediate resistance was found in five strains (55.6%) only to amoxicillin-clavulanic acid. All isolates were susceptible to nalidixic acid, ciprofloxacin, and ceftriaxone, and no ESBL-producing Salmonella were detected. Our findings demonstrated the involvement of three Salmonella serovars in acute gastroenteritis; resistance to penicillins and cephalosporins was common.
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Submitted on : Sunday, October 2, 2016 - 8:02:41 PM
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Casmir Ifeanyichukwu Cajetan Ifeanyi, Bassey Enya Bassey, Nkiruka Florence Ikeneche, Nazek Al-Gallas. Molecular characterization and antibiotic resistance of Salmonellain children with acute gastroenteritis in Abuja, Nigeria. Journal of Infection in Developing Countries, 2014, 8 (06), pp.712-9. ⟨10.3855/jidc.4185⟩. ⟨pasteur-01375049⟩



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