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Journal articles
Process intensification for an enhanced replication of a newly adapted RM-65 sheep pox virus strain in Vero cells grown in stirred bioreactor
Abstract : Sheep pox virus initially adapted to replicate in primary lamb kidney cells was adapted to Vero cells by serial passages in monolayer cultures. After nine passages the virus was able to correctly replicate in Vero cells, virus titer achieved was 10(5.875) TCID50 (median tissue culture infective dose) ml(-1). To optimize the production process, the effects of MO! (multiplicity of infection), TOI (time of infection) and the culture medium were investigated. Cell infection at a MO! of 0.005 concurrently with cell seeding showed the best results in terms of specific virus productivity. The effect of MEM enrichment with several components was investigated using the experimental design approach. 67 experiments were performed in 6-well plates to select the best combination. The highest titer was achieved when MEM was supplemented with 5 mM glucose, 5 mM fructose and 25 mM sucrose. Spinner culture confirms these data; virus titer was 10(7.375) TCID50 ml(-1). In addition Vero cells were cultivated in a 7-1 bioreactor in batch mode on 3 gl(-1) Cytodex1, and infected at cell seeding at a MO! of 0.005. Maximal virus titer was 10(7.275) TCID50 ml(-1). This corresponds to 44-fold factor enhancement compared to spinner cultures conducted in MEM + 2% FCS. (C) 2014 Elsevier B.V. All rights reserved.
https://hal-riip.archives-ouvertes.fr/pasteur-01375060
Contributor : Institut Pasteur Tunis Connect in order to contact the contributor
Submitted on : Sunday, October 2, 2016 - 8:03:02 PM
Last modification on : Monday, October 8, 2018 - 5:44:08 PM
Contributor : Institut Pasteur Tunis Connect in order to contact the contributor
Submitted on : Sunday, October 2, 2016 - 8:03:02 PM
Last modification on : Monday, October 8, 2018 - 5:44:08 PM