Expression of Toll-like Receptor 9 Increases with Progression of Cervical Neoplasia in Tunisian Women - A Comparative Analysis of Condyloma, Cervical Intraepithelial Neoplasia and Invasive Carcinoma

Emna Fehri 1, 2 Emna Ennaifer 1, 2 Monia Ardhaoui 1, 2 Kaouther Ouerhani 1, 2 Thalja Laassili 1, 2 Rahima Bel Haj Rhouma 2 Ikram Guizani 1 Samir Boubaker 1
1 Laboratoire d’Anatomie Pathologique Humaine et Expérimentale
2 LR11IPT04 - Laboratoire d'Epidémiologie Moléculaire et de Pathologie Expérimentale Appliquée aux Maladies Infectieuses
Abstract : Toll-like receptors (TLRs) are expressed in immune and tumor cells and recognize pathogen-associated molecular patterns. Cervical cancer (CC) is directly linked to a persistent infection with high risk human papillomaviruses (HR-HPVs) and could be associated with alteration of TLRs expression. TLR9 plays a key role in the recognition of DNA viruses and better understanding of this signaling pathway in CC could lead to the development of novel immunotherapeutic approaches. The present study was undertaken to determine the level of TLR9 expression in cervical neoplasias from Tunisian women with 53 formalin-fixed and paraffin-embedded specimens, including 22 samples of invasive cervical carcinoma (ICC), 18 of cervical intraepithelial neoplasia (CIN), 7 of condyloma and 6 normal cervical tissues as control cases. Quantification of TLR9 expression was based on scoring four degrees of extent and intensity of immunostaining in squamous epithelial cells. TLR9 expression gradually increased from CIN1 (80% weak intensity) to CIN2 (83.3% moderate), CIN3 (57.1% strong) and ICC (100% very strong). It was absent in normal cervical tissue and weak in 71.4% of condyloma. The mean scores of TLR9 expression were compared using the Kruskall-Wallis test and there was a statistical significance between normal tissue and condyloma as well as between condyloma, CINs and ICC. These results suggest that TLR9 may play a role in progression of cervical neoplasia in Tunisian patients and could represent a useful biomarker for malignant transformation of cervical squamous cells.
Keywords : Toll immunohistochemistry cervical cancer like receptors TLR9
Type de document :
Article dans une revue
Asian Pacific Journal of Cancer Prevention, Asian Pacific Education Press Ltd., 2014, 15 (15), pp.6145-6150. 〈10.7314/APJCP.2014.15.15.6145〉
Liste complète des métadonnées

Littérature citée [56 références]  Voir  Masquer  Télécharger
https://hal-riip.archives-ouvertes.fr/pasteur-01375103
Contributeur : Institut Pasteur Tunis <>
Soumis le : mardi 5 septembre 2017 - 12:06:43
Dernière modification le : lundi 5 février 2018 - 15:22:14
Document(s) archivé(s) le : mercredi 10 janvier 2018 - 10:24:11

Fichier

APJCP_Volume 15_Issue 15_Pages...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

RIIP | RIIP_TUNIS

Citation

Emna Fehri, Emna Ennaifer, Monia Ardhaoui, Kaouther Ouerhani, Thalja Laassili, et al.. Expression of Toll-like Receptor 9 Increases with Progression of Cervical Neoplasia in Tunisian Women - A Comparative Analysis of Condyloma, Cervical Intraepithelial Neoplasia and Invasive Carcinoma. Asian Pacific Journal of Cancer Prevention, Asian Pacific Education Press Ltd., 2014, 15 (15), pp.6145-6150. 〈10.7314/APJCP.2014.15.15.6145〉. 〈pasteur-01375103〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

27

Téléchargements de fichiers

18

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD