Molecular characterization of plasmid-encoded ACC-1a4 beta-lactamase produced by Salmonella strains isolated from water in a Tunisian hospital - Archive ouverte HAL Access content directly
Journal Articles Annals of Microbiology Year : 2012

Molecular characterization of plasmid-encoded ACC-1a4 beta-lactamase produced by Salmonella strains isolated from water in a Tunisian hospital

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Adriana Herteg Fernea
  • Function : Author
Hanene Bouraoui
  • Function : Author
  • PersonId : 976136
Ridha Ben Aissa
  • Function : Author
  • PersonId : 945083
Paola Sandra Mercuri
  • Function : Author

Abstract

A collection of non-typhoidal Salmonella strains, designated MMB1 to MMB18, resistant to third generation cephalosporins were isolated from water in a Tunisian hospital. Isolates harbored a new bla(ACC) gene located on a small transmissible plasmid of 5 kb. The ACC-1a4 enzyme was produced in Escherichia coli, purified and characterized. Compared to the chromosomal bla(ACC-1a) gene, the bla(ACC-1a4) exhibit three mutations. We determined the kinetic parameters of our enzyme for a representative series of beta-lactam substrates. The ACC-1a4 enzyme was active on penicillins and cephalosporins, nitrocefin and cephalothin being its best tested substrates. This is a real cause for alarm since these mutations showed that the DNA sequence of bla(ACC) is an intermediate between those of bla(ACC-1a) and bla(ACC-4) and might increase the probability of the appearance and spreading of ACC-4-mediated resistance in the hospital environment.
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Dates and versions

pasteur-01375141 , version 1 (02-10-2016)

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Melek Ben Aissa, Adriana Herteg Fernea, Hanene Bouraoui, Ridha Ben Aissa, Paola Sandra Mercuri, et al.. Molecular characterization of plasmid-encoded ACC-1a4 beta-lactamase produced by Salmonella strains isolated from water in a Tunisian hospital. Annals of Microbiology, 2012, 62 (1), pp.31-36. ⟨10.1007/s13213-011-0221-x⟩. ⟨pasteur-01375141⟩

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