RT-PCR assays for in vivo expression of Vibrio alginalyticus virulence genes in cultured gilthead Dicentrarchus labrax and Sparus aurata

Abstract : In this study, we investigated the pathogenic potency of two Vibrio alginolyticus strains isolated from cultured gilthead Dicentrarchus labrax and Sparus aurata, in Tunisia. The enzymatic activities of the extracellular products of the studied strains characterized with the API-ZYM system, revealed the existence of several activities. The 50% lethal doses levels were then determined, virulence levels ranged from 1.03 x 10(5) to 1.0 x 10(6) CFU/fish. In addition, we also searched for the presence of eight Vibrio cholerae virulence genes: ctxA, zot, ace, toxR, toxS, toxRS, toxT, and Virulence Pathogenicity Island (VPI), in the genome of two isolated strains of V. alginolitycus. The in vivo expression of toxR, toxS, VPI and ace genes in both fish species, was tested by reverse transcriptase polymerase chain reaction. Indeed, we have noted that for each strain all the virulence genes selected were expressed in sea bream as well as in sea bass.
Type de document :
Article dans une revue
Annals of Microbiology, Springer, 2009, 59 (1), pp.63-67
Liste complète des métadonnées

Littérature citée [20 références]  Voir  Masquer  Télécharger

https://hal-riip.archives-ouvertes.fr/pasteur-01375181
Contributeur : Institut Pasteur Tunis <>
Soumis le : mercredi 7 décembre 2016 - 12:07:50
Dernière modification le : lundi 5 février 2018 - 15:22:12
Document(s) archivé(s) le : mardi 21 mars 2017 - 13:28:49

Fichier

abdallah2009.pdf
Accord explicite pour ce dépôt

Identifiants

  • HAL Id : pasteur-01375181, version 1

Collections

Citation

Fethi Ben Abdallah, Kamel Chaieb, Héla Kallel, Amina Bakhrouf. RT-PCR assays for in vivo expression of Vibrio alginalyticus virulence genes in cultured gilthead Dicentrarchus labrax and Sparus aurata. Annals of Microbiology, Springer, 2009, 59 (1), pp.63-67. 〈pasteur-01375181〉

Partager

Métriques

Consultations de la notice

38

Téléchargements de fichiers

73