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Identification and characterization of a new Leishmania major specific 3'nucleotidase/nuclease protein.

Abstract : We report the characterization of a new Leishmania major gene, lmaj3'nt/nu, encoding a 382 amino acids protein, Lmaj3'NT/NU, that belongs to the 3'nucleotidase/nuclease family. Interestingly, sequence and phylogenetic analysis show that this protein is Leishmania major specific and thus constitutes a new 3'nucleotidase/nuclease subgroup. Lmaj3'NT/NU displays nuclease enzymatic activity and Western blot analysis shows that it is exclusively expressed in promastigotes. Immunofluorescence microscopy using a specific anti-Lmaj3'NT/NU shows that the protein has a plasma membrane localization. Surprisingly, contrary to the previously described Leishmania mexicana 3'NT/NU, lmaj3'nt/nu is not up-regulated when parasites are cultured under purine starvation conditions. Together, these findings suggest Lmaj3'NT/NU may constitute a new important compound of the L. major purine scavenging pathway and could be involved in sandfly parasite survival and colonization.
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https://hal-riip.archives-ouvertes.fr/pasteur-01375213
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Submitted on : Thursday, December 15, 2016 - 10:44:46 AM
Last modification on : Monday, October 8, 2018 - 5:44:09 PM

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Inès Lakhal-Naouar, Yosser Chenik, Yvan Boublik, Mounira Meddeb, Ahlem Aamouri, et al.. Identification and characterization of a new Leishmania major specific 3'nucleotidase/nuclease protein.. Biochemical and Biophysical Research Communications, Elsevier, 2008, 375 (1), pp.54-8. ⟨10.1016/j.bbrc.2008.07.099⟩. ⟨pasteur-01375213⟩

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