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E. Moshkovskii and E. Dispar, A, Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the 18S rRNA gene of E. moshkovskii: Lane PM: 100-basepair (bp) DNA ladder Lanes 1 and 2: E. moshkovskii Laredo strain PCR product undigested and digested with XhoI. Lanes 3?4 and 5?6: PCR products of the two positive samples, Lanes 3 and 4: PCR product of sample (1) undigested and digested with XhoI, Lanes 5 and 6: PCR product of sample (2) undigested and digested with XhoI. Lane 7: negative control. B, PCR analysis of the 18S rRNA gene of E. dispar: Lane PM: 100 (bp) DNA ladder Lane 1: positive control, FIGURE 1. Polymerase chain reaction (PCR) analysis of the 18S ribosomal RNA (rRNA) genes